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MGP003071

UniProt Annotations

Entry Information
Gene Nametubulin folding cofactor D
Protein EntryTBCD_HUMAN
UniProt IDQ9BTW9
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=5; Name=1; IsoId=Q9BTW9-1; Sequence=Displayed; Name=2; IsoId=Q9BTW9-2; Sequence=VSP_017210; Note=No experimental confirmation available.; Name=3; IsoId=Q9BTW9-3; Sequence=VSP_017211, VSP_017215, VSP_017213, VSP_017214; Name=4; IsoId=Q9BTW9-4; Sequence=VSP_017217, VSP_017218; Note=No experimental confirmation available.; Name=5; IsoId=Q9BTW9-5; Sequence=VSP_017212, VSP_017216; Note=No experimental confirmation available.;
FunctionTubulin-folding protein; involved in the first step of the tubulin folding pathway. Modulates microtubule dynamics by capturing GTP-bound beta-tubulin (TUBB). Acts as a GTPase- activating protein (GAP) for ARL2. Its ability to interact with beta tubulin is regulated via its interaction with ARL2. Induces microtubule disruption in absence of ARL2. Increases degradation of beta tubulin, when overexpressed in polarized cells. Promotes epithelial cell detachment, a process antagonized by ARL2. Induces tight adherens and tight junctions disassembly at the lateral cell membrane. {ECO:0000269|PubMed:10722852, ECO:0000269|PubMed:10831612, ECO:0000269|PubMed:11847227, ECO:0000269|PubMed:20740604}.
InductionDown-regulated by shear stress. {ECO:0000269|PubMed:11110777}.
Sequence CautionSequence=AAH39654.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAA76832.2; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
SimilarityBelongs to the TBCD family. {ECO:0000305}.
SimilarityContains 3 HEAT repeats. {ECO:0000305}.
Subcellular LocationCell junction, tight junction {ECO:0000250}. Lateral cell membrane {ECO:0000250}. Cytoplasm {ECO:0000250}. Cell junction, adherens junction {ECO:0000250}. Note=Localized in cell- cell contacts. {ECO:0000250}.
SubunitFound in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. Interacts with PPP2CB. Interacts with ARL2 (By similarity). Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D- tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state. Interacts with ARL2; interaction is enhanced with the GDP- bound form of ARL2. Does not interact with ARL3, ARL4A and ARL4D. Interacts with beta tubulin. {ECO:0000250, ECO:0000269|PubMed:10831612}.
Tissue SpecificityUbiquitously expressed. {ECO:0000269|PubMed:10231032, ECO:0000269|PubMed:11110777}.
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