MGP Database

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MGP003641

UniProt Annotations

Entry Information
Gene Nameeukaryotic translation initiation factor 3, subunit G
Protein EntryEIF3G_HUMAN
UniProt IDO75821
SpeciesHuman
Comments
Comment typeDescription
FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA.
InteractionO95831:AIFM1; NbExp=9; IntAct=EBI-366632, EBI-356440; P55884:EIF3B; NbExp=4; IntAct=EBI-366632, EBI-366696;
Mass SpectrometryMass=35481.1; Mass_error=0.4; Method=MALDI; Range=1-320; Evidence={ECO:0000269|PubMed:18599441};
Mass SpectrometryMass=35639.8; Method=Unknown; Range=1-320; Evidence={ECO:0000269|PubMed:17322308};
PtmPhosphorylated. Phosphorylation is enhanced upon serum stimulation. {ECO:0000255|HAMAP-Rule:MF_03006, ECO:0000269|PubMed:17322308, ECO:0000269|PubMed:18669648, ECO:0000269|PubMed:19690332}.
SimilarityBelongs to the eIF-3 subunit G family. {ECO:0000255|HAMAP-Rule:MF_03006}.
SimilarityContains 1 RRM (RNA recognition motif) domain. {ECO:0000255|HAMAP-Rule:MF_03006}.
Subcellular LocationCytoplasm {ECO:0000255|HAMAP-Rule:MF_03006}. Nucleus {ECO:0000255|HAMAP-Rule:MF_03006, ECO:0000269|PubMed:17094969}. Cytoplasm, perinuclear region {ECO:0000255|HAMAP-Rule:MF_03006, ECO:0000269|PubMed:17094969}. Note=Colocalizes with AIFM1 in the nucleus and perinuclear region.
SubunitComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts (via C-terminus) with AIFM1 (via N-terminus). {ECO:0000255|HAMAP-Rule:MF_03006, ECO:0000269|PubMed:14519125, ECO:0000269|PubMed:14688252, ECO:0000269|PubMed:16766523, ECO:0000269|PubMed:17094969, ECO:0000269|PubMed:17322308, ECO:0000269|PubMed:18599441, ECO:0000269|PubMed:9822659, ECO:0000269|PubMed:9973622}.
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