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MGP003785

UniProt Annotations

Entry Information
Gene Namesolute carrier family 7 (amino acid transporter light chain, y+L system), member 6
Protein EntryYLAT2_HUMAN
UniProt IDQ92536
SpeciesHuman
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=295 uM for glutamine (in the presence of NaCl) {ECO:0000269|PubMed:10903140}; KM=236 uM for leucine (in the presence of NaCl) {ECO:0000269|PubMed:10903140}; KM=120 uM for arginine (in the presence of NaCl) {ECO:0000269|PubMed:10903140}; KM=138 uM for arginine (in the absence of NaCl) {ECO:0000269|PubMed:10903140};
Enzyme RegulationArginine transport is strongly inhibited by lysine, glutamate, leucine, glutamine, methionine and histidine, in the presence of Na(+). Also inhibited by protein kinase C (PKC) and treatment with phorbol-12-myristate-13-acetate (PMA). Mutual inhibition is observed when leucine or glutamine is used as substrate. Inhibition of arginine and leucine uptake also occurs when SLC3A2/4F2hc is either truncated at its C-terminus or mutated at critical residues within the terminal 15 amino acids.
FunctionInvolved in the sodium-independent uptake of dibasic amino acids and sodium-dependent uptake of some neutral amino acids. Requires coexpression with SLC3A2/4F2hc to mediate the uptake of arginine, leucine and glutamine. Also acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Involved in the transport of L-arginine in monocytes. Reduces uptake of ornithine in retinal pigment epithelial (RPE) cells. {ECO:0000269|PubMed:10903140, ECO:0000269|PubMed:11311135, ECO:0000269|PubMed:14603368, ECO:0000269|PubMed:15280038, ECO:0000269|PubMed:16785209, ECO:0000269|PubMed:17197568, ECO:0000269|PubMed:17329401, ECO:0000269|PubMed:9829974}.
Sequence CautionSequence=BAA13376.2; Type=Erroneous initiation; Evidence={ECO:0000305};
SimilarityBelongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family. {ECO:0000255}.
Subcellular LocationBasolateral cell membrane {ECO:0000269|PubMed:15756301, ECO:0000269|PubMed:16785209}; Multi- pass membrane protein {ECO:0000269|PubMed:15756301, ECO:0000269|PubMed:16785209}.
SubunitDisulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc. {ECO:0000269|PubMed:11311135, ECO:0000269|PubMed:16785209}.
Tissue SpecificityExpressed in normal fibroblasts and those from LPI patients. Also expressed in HUVECs, monocytes, RPE cells, and various carcinoma cell lines. {ECO:0000269|PubMed:11078698, ECO:0000269|PubMed:11742806, ECO:0000269|PubMed:14603368, ECO:0000269|PubMed:15280038, ECO:0000269|PubMed:17197568, ECO:0000269|PubMed:17329401}.
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