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MGP003823

UniProt Annotations

Entry Information
Gene Namezw10 kinetochore protein
Protein EntryZW10_HUMAN
UniProt IDO43264
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; IsoId=O43264-1; Sequence=Displayed; Name=2; IsoId=O43264-2; Sequence=VSP_056006; Note=No experimental confirmation available.;
Developmental StageNo significant variation in expression during cell cycle.
FunctionEssential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum. {ECO:0000269|PubMed:11590237, ECO:0000269|PubMed:15029241, ECO:0000269|PubMed:15094189, ECO:0000269|PubMed:15485811, ECO:0000269|PubMed:15824131}.
InteractionO95229:ZWINT; NbExp=6; IntAct=EBI-1001217, EBI-1001132;
MiscellaneousOverexpression as well as silencing of ZW10 disrupts the morphology of the ER-Golgi intermediate compartment as well as the Golgi apparatus and slows down ER-Golgi transport.
SimilarityBelongs to the ZW10 family. {ECO:0000305}.
Subcellular LocationCytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Dynamic pattern of localization during the cell cycle. In most cells at interphase, present diffusely in the cytoplasm. In prometaphase, associated with the kinetochore. At metaphase, detected both at the kinetochores and, most prominently, at the spindle, particularly at the spindle poles. In very early anaphase, detected on segregating kinetochores. In late anaphase and telophase, accumulates at the spindle midzone.
SubunitAssociated with a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L, and SEC22B through direct interaction with RINT1/TIP20L bound to BNIP1/SEC20L. Component of the RZZ complex composed of KNTC1/ROD, ZW10 and ZWILCH. Interacts with C19orf25, KNTC1, and ZWINT. {ECO:0000269|PubMed:12686595, ECO:0000269|PubMed:15029241, ECO:0000269|PubMed:15272311, ECO:0000269|PubMed:15485811, ECO:0000269|PubMed:15824131, ECO:0000269|PubMed:16732327}.
Tissue SpecificityWidely expressed.
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