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MGP003898

UniProt Annotations

Entry Information
Gene Namecarbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2
Protein EntryCHST2_HUMAN
UniProt IDQ9Y4C5
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative initiation; Named isoforms=2; Name=1; IsoId=Q9Y4C5-1; Sequence=Displayed; Name=2; IsoId=Q9Y4C5-2; Sequence=VSP_018887; Note=Higher levels of expression compared to isoform 1 when expressed in HeLa cells. Exhibits similar intracellular GlcNAc-6-O-sulfation activity.;
Biophysicochemical PropertiesKinetic parameters: KM=3.9 uM for PAPS {ECO:0000269|PubMed:12501187}; KM=1.4 mM for BetaBnO-GlcNAc {ECO:0000269|PubMed:12501187};
FunctionSulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains. {ECO:0000269|PubMed:11042394}.
InductionUp-regulated upon cytokine activation. {ECO:0000269|PubMed:11310842}.
PtmGlycosylation at Asn-475 is required for catalytic activity. {ECO:0000269|PubMed:19571171}.
Sequence CautionSequence=BAA34265.2; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAB16886.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAB16887.1; Type=Erroneous initiation; Evidence={ECO:0000305};
SimilarityBelongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily. {ECO:0000305}.
Subcellular LocationGolgi apparatus, trans-Golgi network membrane {ECO:0000269|PubMed:12855678, ECO:0000269|PubMed:22260995}; Single-pass type II membrane protein {ECO:0000269|PubMed:12855678, ECO:0000269|PubMed:22260995}.
SubunitHomodimer; disulfide-linked. Homodimerization is not essential for enzyme activity. {ECO:0000269|PubMed:15220337, ECO:0000269|PubMed:22260995}.
Tissue SpecificityWidely expressed. Highly expressed in bone marrow, peripheral blood leukocytes, spleen, brain, spinal cord, ovary and placenta. Expressed by high endothelial cells (HEVs) and leukocytes. {ECO:0000269|PubMed:10049591, ECO:0000269|PubMed:11310842, ECO:0000269|PubMed:9722682}.
Web ResourceName=GGDB; Note=GlycoGene database; URL="http://jcggdb.jp/rcmg/ggdb/Homolog?cat=symbol&symbol=CHST2";
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