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MGP004052

UniProt Annotations

Entry Information
Gene NamePAN2 poly(A) specific ribonuclease subunit
Protein EntryPAN2_HUMAN
UniProt IDQ504Q3
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q504Q3-1; Sequence=Displayed; Name=2; IsoId=Q504Q3-2; Sequence=VSP_023749; Name=3; IsoId=Q504Q3-3; Sequence=VSP_023748; Note=No experimental confirmation available.;
Catalytic ActivityExonucleolytic cleavage of poly(A) to 5'-AMP. {ECO:0000255|HAMAP-Rule:MF_03182, ECO:0000269|PubMed:14583602}.
CautionHas no ubiquitin carboxyl terminal hydrolase activity since it lacks a conserved catalytic Cys at position 525. {ECO:0000305}.
Enzyme RegulationPositively regulated by the regulatory subunit PAN3. {ECO:0000255|HAMAP-Rule:MF_03182}.
FunctionCatalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA when the poly(A) stretch is bound by poly(A)-binding protein (PABP), which is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. {ECO:0000255|HAMAP-Rule:MF_03182, ECO:0000269|PubMed:14583602, ECO:0000269|PubMed:16284618}.
InteractionQ58A45:PAN3; NbExp=6; IntAct=EBI-1058976, EBI-2513054;
Sequence CautionSequence=BAA31685.2; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
SimilarityBelongs to the peptidase C19 family. PAN2 subfamily. {ECO:0000255|HAMAP-Rule:MF_03182}.
SimilarityContains 1 exonuclease domain. {ECO:0000255|HAMAP- Rule:MF_03182}.
SimilarityContains 1 USP domain. {ECO:0000255|HAMAP- Rule:MF_03182}.
SimilarityContains 4 WD repeats. {ECO:0000255|HAMAP- Rule:MF_03182}.
Subcellular LocationCytoplasm, P-body. Nucleus. Note=Shuttles between nucleus and cytoplasm.
SubunitInteracts with the regulatory subunit PAN3 to form the poly(A)-nuclease (PAN) deadenylation complex. {ECO:0000255|HAMAP- Rule:MF_03182, ECO:0000269|PubMed:14583602, ECO:0000269|PubMed:23932717}.
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