MGP Database

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MGP004775

UniProt Annotations

Entry Information
Gene Namepolymerase (RNA) I polypeptide A, 194kDa
Protein EntryRPA1_HUMAN
UniProt IDO95602
SpeciesHuman
Comments
Comment typeDescription
Catalytic ActivityNucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). {ECO:0000250}.
InteractionP04626:ERBB2; NbExp=16; IntAct=EBI-359472, EBI-641062; Q9H9Y6:POLR1B; NbExp=2; IntAct=EBI-359472, EBI-355441;
Sequence CautionSequence=AAC99959.1; Type=Frameshift; Positions=1114, 1469; Evidence={ECO:0000305}; Sequence=AAD09356.1; Type=Frameshift; Positions=1114; Evidence={ECO:0000305};
SimilarityBelongs to the RNA polymerase beta' chain family. {ECO:0000305}.
Subcellular LocationNucleus, nucleolus {ECO:0000250}.
SubunitComponent of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with MYO1C. Interacts with ERBB2 (By similarity). {ECO:0000250}.
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