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MGP005393

UniProt Annotations

Entry Information
Gene NameNAD synthetase 1
Protein EntryNADE_HUMAN
UniProt IDQ6IA69
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q6IA69-1; Sequence=Displayed; Name=2; IsoId=Q6IA69-2; Sequence=VSP_056585, VSP_056586; Note=No experimental confirmation available.;
Biophysicochemical PropertiesKinetic parameters: KM=0.49 mM for deamido-NAD(+) {ECO:0000269|PubMed:12547821}; KM=0.089 mM for ATP {ECO:0000269|PubMed:12547821}; KM=1.44 mM for glutamine {ECO:0000269|PubMed:12547821}; KM=13.1 mM for ammonium {ECO:0000269|PubMed:12547821}; Vmax=0.99 nmol/min/ug enzyme deamido-NAD(+) {ECO:0000269|PubMed:12547821}; Vmax=0.61 nmol/min/ug enzyme ATP {ECO:0000269|PubMed:12547821}; Vmax=0.70 nmol/min/ug enzyme glutamine {ECO:0000269|PubMed:12547821}; Vmax=1.04 nmol/min/ug enzyme ammonium {ECO:0000269|PubMed:12547821};
Catalytic ActivityATP + deamido-NAD(+) + L-glutamine + H(2)O = AMP + diphosphate + NAD(+) + L-glutamate.
PathwayCofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.
SimilarityContains 1 CN hydrolase domain. {ECO:0000255|PROSITE- ProRule:PRU00054}.
SimilarityIn the C-terminal section; belongs to the NAD synthetase family. {ECO:0000305}.
SubunitHomohexamer. {ECO:0000269|PubMed:12547821}.
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