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MGP005416

UniProt Annotations

Entry Information
Gene Nameriboflavin kinase
Protein EntryRIFK_HUMAN
UniProt IDQ969G6
SpeciesHuman
Comments
Comment typeDescription
Catalytic ActivityATP + riboflavin = ADP + FMN.
CofactorName=Zn(2+); Xref=ChEBI:CHEBI:29105; Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Zinc or magnesium.;
FunctionCatalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase. {ECO:0000269|PubMed:19641494}.
PathwayCofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.
Sequence CautionSequence=AAH07069.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=BAA92033.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
Subcellular LocationCytoplasm {ECO:0000250}.
SubunitMonomer. Directly interacts with TNFRSF1A death domain. TNFRSF1A-binding may be supported by TRADD. In the absence of TNFRSF1A, interacts with TRADD. Independently of TNFRSF1A, interacts with the NADPH oxidase subunit CYBA. {ECO:0000269|PubMed:12623014, ECO:0000269|PubMed:19641494}.
Tissue SpecificityDetected in brain, placenta and urinary bladder.
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