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MGP005441

UniProt Annotations

Entry Information
Gene Namepolypeptide N-acetylgalactosaminyltransferase 10
Protein EntryGLT10_HUMAN
UniProt IDQ86SR1
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=4; Name=1; IsoId=Q86SR1-1; Sequence=Displayed; Name=2; IsoId=Q86SR1-2; Sequence=VSP_011209; Note=No experimental confirmation available.; Name=3; IsoId=Q86SR1-3; Sequence=VSP_011212, VSP_011213; Note=No experimental confirmation available.; Name=4; IsoId=Q86SR1-4; Sequence=VSP_011207, VSP_011208, VSP_011214; Note=No experimental confirmation available.;
Catalytic ActivityUDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide. {ECO:0000269|PubMed:12417297}.
CautionAccording to experiments made in rat, this enzyme is unable to transfer GalNAc onto serine or threonine residue on the protein receptor, but instead requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties, thereby acting as a glycopeptide transferase. {ECO:0000305}.
CofactorName=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
DomainThere are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding. {ECO:0000250}.
DomainThe ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity. {ECO:0000250}.
FunctionCatalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D- galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward Muc5Ac and EA2 peptide substrates.
PathwayProtein modification; protein glycosylation.
Sequence CautionSequence=BAB14676.1; Type=Erroneous initiation; Evidence={ECO:0000305};
SimilarityBelongs to the glycosyltransferase 2 family. GalNAc-T subfamily. {ECO:0000305}.
SimilarityContains 1 ricin B-type lectin domain. {ECO:0000255|PROSITE-ProRule:PRU00174}.
Subcellular LocationGolgi apparatus membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}.
Tissue SpecificityWidely expressed. Expressed at high level in small intestine, and at intermediate levels in stomach, pancreas, ovary, thyroid gland and spleen. Weakly expressed in other tissues. {ECO:0000269|PubMed:12417297}.
Web ResourceName=Functional Glycomics Gateway - GTase; Note=Polypeptide N-acetylgalactosaminyltransferase 10; URL="http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_492";
Web ResourceName=Functional Glycomics Gateway - GTase; Note=Polypeptide N-acetylgalactosaminyltransferase 10; URL="http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_493";
Web ResourceName=GGDB; Note=GlycoGene database; URL="http://jcggdb.jp/rcmg/ggdb/Homolog?cat=symbol&symbol=GALNT10";
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