Log in / Register

MGP Database

New search | Record Overview | Ontology/Pathway Information | Domain Information | UniProt Annotations | Related Proteins

MGP005849

UniProt Annotations

Entry Information

Gene Name	transient receptor potential cation channel, subfamily M, member 8
Protein Entry	TRPM8_HUMAN
UniProt ID	Q7Z2W7
Species	Human

Comments

Comment type	Description
Alternative Products	Event=Alternative splicing; Named isoforms=4; Name=1; IsoId=Q7Z2W7-1; Sequence=Displayed; Name=2; Synonyms=sTRPM8-18, sM8-18; IsoId=Q7Z2W7-2; Sequence=VSP_053268, VSP_012332, VSP_012333; Name=3; Synonyms=sTRPM8-6, sM8-6; IsoId=Q7Z2W7-3; Sequence=VSP_012330, VSP_012332, VSP_012333; Name=4; IsoId=Q7Z2W7-4; Sequence=VSP_054505, VSP_054506, VSP_054507; Note=No experimental confirmation available.;
Caution	The 192-residue sequence submitted as AAS45276 has been confirmed by the authors of PubMed:22128173 to be incorrect as translation starts from a downstream methionine. {ECO:0000305}.
Domain	The coiled coil region is required for multimerization.
Function	Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonist menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. In prostate cancer cells, shows strong inward rectification and high calcium selectivity in contrast to its behavior in normal cells which is characterized by outward rectification and poor cationic selectivity. Isoform 2 and isoform 3 negatively regulate menthol- and cold-induced channel activity by stabilizing the closed state of the channel. {ECO:0000269\|PubMed:15306801, ECO:0000269\|PubMed:16174775, ECO:0000269\|PubMed:22128173}.
Miscellaneous	Its expression in most prostate tumors as well as the presence of an immunogenic epitope suggest that it may be suitable for the design of peptide vaccination strategies for prostate cancers.
Miscellaneous	The sensation of coolness triggered by eucalyptol or menthol may be explained by the fact that menthol and cool temperatures sensations are detected by this protein.
Sequence Caution	Sequence=AAH01135.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=AAS45276.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
Similarity	Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily. {ECO:0000305}.
Subcellular Location	Cell membrane; Multi-pass membrane protein. Membrane raft. Endoplasmic reticulum membrane. Note=Localizes to membrane rafts but is also located in the cell membrane outside of these regions where channel response to cold is enhanced compared to membrane rafts (By similarity). Located in the endoplasmic reticulum in prostate cancer cells. {ECO:0000250}.
Subunit	Homotetramer (By similarity). Isoform 2 and isoform 3 interact with the C-terminus of isoform 1 in a thermosensitive manner with decreased interaction at 21 degrees Celsius compared to 37 degrees Celsius. {ECO:0000250, ECO:0000269\|PubMed:22128173}.
Tissue Specificity	Expressed in prostate. Also expressed in prostate tumors and in non-prostatic primary tumors such as colon, lung, breast and skin tumors. {ECO:0000269\|PubMed:11325849, ECO:0000269\|PubMed:12858355, ECO:0000269\|PubMed:16174775, ECO:0000269\|PubMed:22128173}.