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MGP006864

UniProt Annotations

Entry Information
Gene Namelysocardiolipin acyltransferase 1
Protein Entry
UniProt ID
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q6UWP7-1; Sequence=Displayed; Name=2; IsoId=Q6UWP7-2; Sequence=VSP_026181, VSP_026182; Note=No experimental confirmation available.; Name=3; IsoId=Q6UWP7-3; Sequence=VSP_044307;
Biophysicochemical PropertiesKinetic parameters: KM=39 uM for arachidonoyl-CoA (20:4) for LPIAT activity {ECO:0000269|PubMed:19075029}; KM=289 uM for oleoyl-CoA (18:1) for LPIAT activity {ECO:0000269|PubMed:19075029}; KM=134 uM for linoleoyl-CoA (18:2) for LPIAT activity {ECO:0000269|PubMed:19075029}; KM=34 uM for stearoyl-CoA (18:0) for LPIAT activity {ECO:0000269|PubMed:19075029}; KM=53 uM for palmitoyl-CoA (16:0) for LPIAT activity {ECO:0000269|PubMed:19075029}; KM=54 uM for oleoyl-CoA (18:1) for LPGAT activity {ECO:0000269|PubMed:19075029}; KM=36 uM for linoleoyl-CoA (18:2) for LPGAT activity {ECO:0000269|PubMed:19075029}; KM=70 uM for stearoyl-CoA (18:0) for LPGAT activity {ECO:0000269|PubMed:19075029}; KM=34 uM for palmitoyl-CoA (16:0) for LPGAT activity {ECO:0000269|PubMed:19075029}; Vmax=3230 nmol/min/mg enzyme toward arachidonoyl-CoA (20:4) for LPIAT activity {ECO:0000269|PubMed:19075029}; Vmax=7489 nmol/min/mg enzyme toward oleoyl-CoA (18:1) for LPIAT activity {ECO:0000269|PubMed:19075029}; Vmax=4696 nmol/min/mg enzyme toward linoleoyl-CoA (18:2) for LPIAT activity {ECO:0000269|PubMed:19075029}; Vmax=1078 nmol/min/mg enzyme toward stearoyl-CoA (18:0) for LPIAT activity {ECO:0000269|PubMed:19075029}; Vmax=11203 nmol/min/mg enzyme toward palmitoyl-CoA (16:0) for LPIAT activity {ECO:0000269|PubMed:19075029}; Vmax=5514 nmol/min/mg enzyme toward oleoyl-CoA (18:1) for LPGAT activity {ECO:0000269|PubMed:19075029}; Vmax=1358 nmol/min/mg enzyme toward linoleoyl-CoA (18:2) for LPGAT activity {ECO:0000269|PubMed:19075029}; Vmax=3530 nmol/min/mg enzyme toward stearoyl-CoA (18:0) for LPGAT activity {ECO:0000269|PubMed:19075029}; Vmax=1673 nmol/min/mg enzyme toward palmitoyl-CoA (16:0) for LPGAT activity {ECO:0000269|PubMed:19075029};
Catalytic ActivityAcyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.
CautionPubMed:16620771 does not detect acyl-CoA:lysocardiolipin acyltransferase activity. {ECO:0000305}.
DomainThe HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. {ECO:0000250}.
FunctionAcyl-CoA:lysocardiolipin acyltransferase. Possesses both lysophosphatidylinositol acyltransferase (LPIAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities. Recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors. Acts as a remodeling enzyme for cardiolipin, a major membrane polyglycerophospholipid. Converts lysophosphatidic acid (LPA) into phosphatidic acid (PA) with a relatively low activity. Required for establishment of the hematopoietic and endothelial lineages. {ECO:0000269|PubMed:16620771, ECO:0000269|PubMed:19075029}.
PathwayPhospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
SimilarityBelongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum membrane {ECO:0000269|PubMed:19075029}; Multi-pass membrane protein {ECO:0000269|PubMed:19075029}.
Tissue SpecificityExpressed at higher level in heart, kidney and pancreas than in brain, spleen, liver, lung, small intestine and placenta. {ECO:0000269|PubMed:16620771, ECO:0000269|PubMed:19075029}.
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