MGP Database

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MGP007071

UniProt Annotations

Entry Information
Gene Nameectonucleoside triphosphate diphosphohydrolase 8
Protein EntryENTP8_HUMAN
UniProt IDQ5MY95
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q5MY95-1; Sequence=Displayed; Name=2; IsoId=Q5MY95-2; Sequence=VSP_028559;
Biophysicochemical PropertiesKinetic parameters: KM=81 uM for ATP {ECO:0000269|PubMed:17095758}; KM=137 uM for ADP {ECO:0000269|PubMed:17095758}; KM=480 uM for UTP {ECO:0000269|PubMed:17095758}; KM=241 uM for UDP {ECO:0000269|PubMed:17095758}; Vmax=790 nmol/min/mg enzyme with ATP as substrate {ECO:0000269|PubMed:17095758}; Vmax=163 nmol/min/mg enzyme with ADP as substrate {ECO:0000269|PubMed:17095758}; Vmax=1100 nmol/min/mg enzyme with UTP as substrate {ECO:0000269|PubMed:17095758}; Vmax=110 nmol/min/mg enzyme with UDP as substrate {ECO:0000269|PubMed:17095758};
Catalytic ActivityA nucleoside 5'-triphosphate + 2 H(2)O = a nucleoside 5'-phosphate + 2 phosphate. {ECO:0000269|PubMed:16752921, ECO:0000269|PubMed:17095758}.
CofactorName=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250}; Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Note=Ca(2+) or Mg(2+). Has lower efficiency with Mg(2+). {ECO:0000250};
DomainThe transmembranous domains are involved in regulation of enzyme activity. {ECO:0000269|PubMed:16752921}.
Enzyme RegulationNot inhibited by ARL 67156. {ECO:0000269|PubMed:17603550}.
FunctionCanalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolysis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP. {ECO:0000269|PubMed:16752921, ECO:0000269|PubMed:17095758}.
PtmN-glycosylated. {ECO:0000269|PubMed:16752921}.
SimilarityBelongs to the GDA1/CD39 NTPase family. {ECO:0000305}.
Subcellular LocationCell membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
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