Summary of Study ST002476

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001599. The data can be accessed directly via it's Project DOI: 10.21228/M8113P This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002476
Study TitleHigh body temperature increases gut microbiota-dependent host resistance to influenza A virus and SARS-CoV-2 infection (Mouse)
Study SummaryWhile a common symptom of influenza and coronavirus disease 2019 (COVID-19) is fever, its physiological role on host resistance to viral infection remains less clear. Here, we demonstrate that exposure of mice to the high ambient temperature of 36 °C increase host resistance to viral pathogens including influenza virus and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). High heat-exposed mice increase basal body temperature over 38 °C to enable more bile acids production in a gut microbiota-dependent manner. The gut microbiota-derived deoxycholic acid (DCA) and its plasma membrane-bound receptor Takeda G-protein-coupled receptor 5 (TGR5) signaling increase host resistance to influenza virus infection by suppressing virus replication and neutrophil-dependent tissue damage. Furthermore, the DCA and its nuclear farnesoid X receptor (FXR) agonist protect Syrian hamster from lethal SARS-CoV-2 infection. Moreover, we demonstrate that certain bile acids are reduced in the plasma of COVID-19 patients who developed moderate I/II disease compared with minor illness group. These findings uncover an unexpected mechanism by which virus-induced high fever increases host resistance to influenza virus and SARS-CoV-2 in a gut microbiota-dependent manner.
Institute
Keio University
Last NameFukuda
First NameShinji
AddressKakuganji 246-2, Mizukami, Tsuruoka City Yamagata,Japan
Emailsfukuda@sfc.keio.ac.jp
Phone+81-235-29-0528
Submit Date2023-01-24
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailCE-MS
Release Date2023-03-10
Release Version2
Shinji Fukuda Shinji Fukuda
https://dx.doi.org/10.21228/M8113P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR001599
Project DOI:doi: 10.21228/M8113P
Project Title:High body temperature increases gut microbiota-dependent host resistance to influenza A virus and SARS-CoV-2 infection
Project Summary:While a common symptom of influenza and coronavirus disease 2019 (COVID-19) is fever, its physiological role on host resistance to viral infection remains less clear. Here, we demonstrate that exposure of mice to the high ambient temperature of 36 °C increase host resistance to viral pathogens including influenza virus and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). High heat-exposed mice increase basal body temperature over 38 °C to enable more bile acids production in a gut microbiota-dependent manner. The gut microbiota-derived deoxycholic acid (DCA) and its plasma membrane-bound receptor Takeda G-protein-coupled receptor 5 (TGR5) signaling increase host resistance to influenza virus infection by suppressing virus replication and neutrophil-dependent tissue damage. Furthermore, the DCA and its nuclear farnesoid X receptor (FXR) agonist protect Syrian hamster from lethal SARS-CoV-2 infection. Moreover, we demonstrate that certain bile acids are reduced in the plasma of COVID-19 patients who developed moderate I/II disease compared with minor illness group. These findings uncover an unexpected mechanism by which virus-induced high fever increases host resistance to influenza virus and SARS-CoV-2 in a gut microbiota-dependent manner.
Institute:Keio University
Last Name:Fukuda
First Name:Shinji
Address:246-2 Mizukami, Kakuganji, Tsuruoka-city, Yamagata, 997-0052, Japan
Email:sfukuda@sfc.keio.ac.jp
Phone:+81-235-29-0528

Subject:

Subject ID:SU002584
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Temp/Duration
SA2490281422C for 7 d
SA2490291322C for 7 d
SA2490301222C for 7 d
SA2490311522C for 7 d
SA2490321822C for 7 d
SA2490332022C for 7 d
SA2490341922C for 7 d
SA2490351122C for 7 d
SA2490361722C for 7 d
SA2490371622C for 7 d
SA2490382536C for 7 d
SA2490392436C for 7 d
SA2490402336C for 7 d
SA2490412636C for 7 d
SA2490422736C for 7 d
SA2490433036C for 7 d
SA2490442936C for 7 d
SA2490452836C for 7 d
SA2490462236C for 7 d
SA2490472136C for 7 d
SA24904874C for 7 d
SA24904984C for 7 d
SA24905094C for 7 d
SA24905124C for 7 d
SA24905214C for 7 d
SA24905364C for 7 d
SA24905434C for 7 d
SA24905544C for 7 d
SA24905654C for 7 d
SA249057104C for 7 d
Showing results 1 to 30 of 30

Collection:

Collection ID:CO002577
Collection Summary:Cecal contents were obtained by dissection.
Sample Type:cecal contents

Treatment:

Treatment ID:TR002596
Treatment Summary:NA

Sample Preparation:

Sampleprep ID:SP002590
Sampleprep Summary:Cecal metabolites were extracted by vigorous shaking with methanol containing methionine sulfone and D-camphol-10-sulfonic acid as the internal standards.

Combined analysis:

Analysis ID AN004077 AN004078
Analysis type MS MS
Chromatography type CE CE
Chromatography system Agilent CE Agilent CE
Column Fused-silica, i.d. 50 µm(cation) COSMO(+), i.d.50 μm(anion)
MS Type ESI ESI
MS instrument type TOF TOF
MS instrument name Agilent CE-TOFMS Agilent CE-TOFMS
Ion Mode POSITIVE NEGATIVE
Units nmol/g nmol/g

Chromatography:

Chromatography ID:CH003019
Instrument Name:Agilent CE
Column Name:Fused-silica, i.d. 50 µm(cation)
Column Temperature:none
Flow Gradient:none
Flow Rate:none
Solvent A:none
Solvent B:none
Chromatography Type:CE
  
Chromatography ID:CH003020
Instrument Name:Agilent CE
Column Name:COSMO(+), i.d.50 μm(anion)
Column Temperature:none
Flow Gradient:none
Flow Rate:none
Solvent A:none
Solvent B:none
Chromatography Type:CE

MS:

MS ID:MS003824
Analysis ID:AN004077
Instrument Name:Agilent CE-TOFMS
Instrument Type:TOF
MS Type:ESI
MS Comments:In-house software (MasterHands) was used for peak area integration and annotation.
Ion Mode:POSITIVE
  
MS ID:MS003825
Analysis ID:AN004078
Instrument Name:Agilent CE-TOFMS
Instrument Type:TOF
MS Type:ESI
MS Comments:In-house software (MasterHands) was used for peak area integration and annotation.
Ion Mode:NEGATIVE
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