Summary of Study ST001820
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001150. The data can be accessed directly via it's Project DOI: 10.21228/M82690 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001820 |
Study Title | WT neurons treated with APOE3/3 and APOE4/4 ACM |
Study Summary | We performed a targeted lipidomic analysis on WT neurons treated with astrocyte conditioned media (ACM) from APOE3/3 or APOE4/4 astrocytes. |
Institute | Columbia University |
Last Name | Nuriel |
First Name | Tal |
Address | 630 W 168th St., P&S 12-430 |
tn2283@cumc.columbia.edu | |
Phone | 2123045683 |
Submit Date | 2021-06-02 |
Analysis Type Detail | LC-MS |
Release Date | 2021-06-10 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002954 | AN002955 | AN002956 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | HILIC |
Chromatography system | Agilent 1260 | Agilent 1260 | Agilent 1260 |
Column | Agilent Eclipse XDB-C18 (100 x 3.0mm) | Agilent Eclipse XDB-C18 (100 x 3.0mm) | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
MS Type | ESI | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole |
MS instrument name | Agilent 6490 QQQ | Agilent 6490 QQQ | Agilent 6490 QQQ |
Ion Mode | NEGATIVE | POSITIVE | POSITIVE |
Units | area under the curve | area under the curve | area under the curve |
MS:
MS ID: | MS002744 |
Analysis ID: | AN002954 |
Instrument Name: | Agilent 6490 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | Lipid profiling was performed using an Agilent 1260 HPLC coupled to an Agilent 6490 triple quadrupole (QQQ) mass spectrometer (41). Each sample was run through three separate chromatographic conditions (reverse-phase negative mode, reverse-phase positive mode and normal-phase positive mode) for the effective quantification of over 337 distinct lipids from over 28 lipid subclasses, as previously described (41). Individual lipid species were measured by multiple reaction monitoring transitions. |
Ion Mode: | NEGATIVE |
MS ID: | MS002745 |
Analysis ID: | AN002955 |
Instrument Name: | Agilent 6490 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | Lipid profiling was performed using an Agilent 1260 HPLC coupled to an Agilent 6490 triple quadrupole (QQQ) mass spectrometer (41). Each sample was run through three separate chromatographic conditions (reverse-phase negative mode, reverse-phase positive mode and normal-phase positive mode) for the effective quantification of over 337 distinct lipids from over 28 lipid subclasses, as previously described (41). Individual lipid species were measured by multiple reaction monitoring transitions. |
Ion Mode: | POSITIVE |
MS ID: | MS002746 |
Analysis ID: | AN002956 |
Instrument Name: | Agilent 6490 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | Lipid profiling was performed using an Agilent 1260 HPLC coupled to an Agilent 6490 triple quadrupole (QQQ) mass spectrometer (41). Each sample was run through three separate chromatographic conditions (reverse-phase negative mode, reverse-phase positive mode and normal-phase positive mode) for the effective quantification of over 337 distinct lipids from over 28 lipid subclasses, as previously described (41). Individual lipid species were measured by multiple reaction monitoring transitions. |
Ion Mode: | POSITIVE |