Summary of study ST001820

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001150. The data can be accessed directly via it's Project DOI: 10.21228/M82690 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001820
Study TitleWT neurons treated with APOE3/3 and APOE4/4 ACM
Study SummaryWe performed a targeted lipidomic analysis on WT neurons treated with astrocyte conditioned media (ACM) from APOE3/3 or APOE4/4 astrocytes.
Institute
Columbia University
Last NameNuriel
First NameTal
Address630 W 168th St., P&S 12-430
Emailtn2283@cumc.columbia.edu
Phone2123045683
Submit Date2021-06-02
Analysis Type DetailLC-MS
Release Date2021-06-10
Release Version1
Tal Nuriel Tal Nuriel
https://dx.doi.org/10.21228/M82690
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO001890
Collection Summary:Astrocyte conditioned media (ACM) was obtained from immortalized astrocyte cell lines (a gift from Dr. David Holtzman) that were originally generated from primary astrocytes from P1-2 pups of APOE targeted replacement mice (42). The immortalized astrocytes were conditioned with Neurobasal media supplemented with B27, Glutamax-I, Normocin and 1% penicillin/streptomycin for 24 hours. This ACM was then collected, stored at -80ºC, and thawed prior to use. WT primary cortical neuronal cultures were obtained from embryonic day 17 (E17) C57Bl/6 embryos. Briefly, pregnant mice were euthanized by cervical dislocation and the embryo brains extracted. The meninges were carefully stripped off and the cortices dissected out. The cortices were then enzymatically dissociated in 0.25% Trypsin-EDTA and resuspended in Neurobasal media supplemented with B27, Glutamax-I, Normocin and 1% penicillin/streptomycin. Dissociated cells were counted and 300K neurons per well were plated directly into ACM in poly-D-lysine (PDL)-coated 6-well plates. ACM-treated neurons were then incubated at 37⁰C for 7 days in a humidified chamber with 5% CO2, with 50% media exchange (with newly thawed ACM) once every 3 days.
Sample Type:Neurons
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