Summary of Study ST002568
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001655. The data can be accessed directly via it's Project DOI: 10.21228/M8S726 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002568 |
| Study Title | Assessment of metabolic changes in different animal models of maladaptive right ventricular hypertrophy in chronic pulmonary hypertension by lipidomics and HILIC |
| Study Summary | We analyzed plasma samples from 33 Yucatan pigs belonging to four different experimental models of pulmonary hypertension (PH): M1 chronic postcapillary PH by pulmonary vein banding; M2 chronic PH by aorto-pulmonary shunting; M3: right ventricular pressure overload by pulmonary artery banding, thus without PH; and M0 sham procedure. Briefly, blood samples were collected 8 months after surgery and untargeted lipidomics and HILIC metabolomic analyses were performed for negative and positive polarities. For quality control, 5 QC samples were included in each analysis, iterative MSMS was performed for metabolite annotation. Plasma metabolomic patterns differed among groups, displaying arginine-nitric oxide and histidine deficiency in both PH models (M1 and M2), altered taurine and purine pathways in M2, and lipidomic changes in all three models of pressure overload. |
| Institute | Universidad CEU San Pablo |
| Last Name | Moran |
| First Name | Maria |
| Address | Universidad CEU-San Pablo. Urb. Montepríncipe |
| maria.morangarrido@ceu.es | |
| Phone | 913724769 |
| Submit Date | 2023-03-23 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-05-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004229 | AN004230 | AN004231 | AN004232 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC |
| Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
| Column | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) | Waters XBridge BEH Amide (100 x 2.1mm,2.5um) | Waters XBridge BEH Amide (100 x 2.1mm,2.5um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | QTOF | QTOF | QTOF | QTOF |
| MS instrument name | Agilent 6545 QTOF | Agilent 6545 QTOF | Agilent 6560 Ion Mobility | Agilent 6545 QTOF |
| Ion Mode | POSITIVE | NEGATIVE | NEGATIVE | POSITIVE |
| Units | Peak area | Peak area | Peak area | Peak area |
MS:
| MS ID: | MS003976 |
| Analysis ID: | AN004229 |
| Instrument Name: | Agilent 6545 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | the gas temperature in the ion source was 200 ºC, the flow was 10 L/min, the pressure in the nebulizer was 50 psig, the temperature of the sheath gas was 300 ºC and the flow of the sheath gas was 12 L/min. The voltage of the capillary was set at 3500 V, the nozzle voltage was 0 L/min, the fragmentor was set at 150 V in the 6545 system and at 400 V in the 6560 system, the voltage in the skimmer was 65 V and the OctopoleRFPeak was 750. After data acquisition, the data files were inspected using Agilent MassHunter Qualitative 10. MassHunter Profinder 10 was used for alignment of data, the deconvolution process and peak integration. MassHunter Lipid Annotator was used to assist in the lipid identification process, features were tentatively annotated based on the MS1 data using the online toll CEU Mass Mediator. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003977 |
| Analysis ID: | AN004230 |
| Instrument Name: | Agilent 6545 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | the gas temperature in the ion source was 200 ºC, the flow was 10 L/min, the pressure in the nebulizer was 50 psig, the temperature of the sheath gas was 300 ºC and the flow of the sheath gas was 12 L/min. The voltage of the capillary was set at 3000 V, the nozzle voltage was 0 L/min, the fragmentor was set at 150 V in the 6545 system and at 400 V in the 6560 system, the voltage in the skimmer was 65 V and the OctopoleRFPeak was 750.After data acquisition, the data files were inspected using Agilent MassHunter Qualitative 10. MassHunter Profinder 10 was used for alignment of data, the deconvolution process and peak integration. MassHunter Lipid Annotator was used to assist in the lipid identification process, features were tentatively annotated based on the MS1 data using the online toll CEU Mass Mediator. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS003978 |
| Analysis ID: | AN004231 |
| Instrument Name: | Agilent 6560 Ion Mobility |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The gas temperature in the ion source was 225 ºC, the flow was 13 L/min, the pressure in the nebulizer was 35 psig, the temperature of the sheath gas was 350 ºC and the flow of the sheath gas was 12 L/min. The voltage of the capillary was set at 3500 V, the nozzle voltage was 0 L/min, the fragmentor was set at 125 V in the 6545 system and at 400 V in the 6560 system, the voltage in the skimmer was 45 V and the OctopoleRFPeak was 750. After data acquisition, the data files were inspected using Agilent MassHunter Qualitative 10. MassHunter Profinder 10 was used for alignment of data, the deconvolution process and peak integration. Features were tentatively annotated based on the MS1 data using the online toll CEU Mass Mediator. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS003979 |
| Analysis ID: | AN004232 |
| Instrument Name: | Agilent 6545 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The gas temperature in the ion source was 225 ºC, the flow was 13 L/min, the pressure in the nebulizer was 35 psig, the temperature of the sheath gas was 350 ºC and the flow of the sheath gas was 12 L/min. The voltage of the capillary was set at 3500 V, the nozzle voltage was 0 L/min, the fragmentor was set at 125 V in the 6545 system and at 400 V in the 6560 system, the voltage in the skimmer was 45 V and the OctopoleRFPeak was 750. Features were tentatively annotated based on the MS1 data using the online toll CEU Mass Mediator. |
| Ion Mode: | POSITIVE |
