Summary of Study ST002568
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001655. The data can be accessed directly via it's Project DOI: 10.21228/M8S726 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002568 |
Study Title | Assessment of metabolic changes in different animal models of maladaptive right ventricular hypertrophy in chronic pulmonary hypertension by lipidomics and HILIC |
Study Summary | We analyzed plasma samples from 33 Yucatan pigs belonging to four different experimental models of pulmonary hypertension (PH): M1 chronic postcapillary PH by pulmonary vein banding; M2 chronic PH by aorto-pulmonary shunting; M3: right ventricular pressure overload by pulmonary artery banding, thus without PH; and M0 sham procedure. Briefly, blood samples were collected 8 months after surgery and untargeted lipidomics and HILIC metabolomic analyses were performed for negative and positive polarities. For quality control, 5 QC samples were included in each analysis, iterative MSMS was performed for metabolite annotation. Plasma metabolomic patterns differed among groups, displaying arginine-nitric oxide and histidine deficiency in both PH models (M1 and M2), altered taurine and purine pathways in M2, and lipidomic changes in all three models of pressure overload. |
Institute | Universidad CEU San Pablo |
Last Name | Moran |
First Name | Maria |
Address | Universidad CEU-San Pablo. Urb. Montepríncipe |
maria.morangarrido@ceu.es | |
Phone | 913724769 |
Submit Date | 2023-03-23 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2023-05-10 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004229 | AN004230 | AN004231 | AN004232 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC |
Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
Column | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) | Waters XBridge BEH Amide (100 x 2.1mm,2.5um) | Waters XBridge BEH Amide (100 x 2.1mm,2.5um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | QTOF | QTOF | QTOF | QTOF |
MS instrument name | Agilent 6545 QTOF | Agilent 6545 QTOF | Agilent 6560 Ion Mobility | Agilent 6545 QTOF |
Ion Mode | POSITIVE | NEGATIVE | NEGATIVE | POSITIVE |
Units | Peak area | Peak area | Peak area | Peak area |
Chromatography:
Chromatography ID: | CH003138 |
Instrument Name: | Agilent 1290 Infinity II |
Column Name: | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) |
Column Temperature: | 50 °C |
Flow Gradient: | 0.0-1.0 min: 70% B, 1.0-3.5 min: 86% B, 3.5-10 min: 86% B, 10.0-11.0 min: 100% B, 11.0-17.0 100% B, 17.0-19.0 min: 70% B |
Flow Rate: | 0.6 mL/min |
Solvent A: | 90% water/1% methanol, 10 mM ammonium acetate, 0.2 mM ammonium fluoride |
Solvent B: | 20% acetonitrile/30% methanol/50% isopropanol, 10 mM ammonium acetate, 0.2 mM ammonium fluoride |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003139 |
Instrument Name: | Agilent 1290 Infinity II |
Column Name: | Waters XBridge BEH Amide (100 x 2.1mm,2.5um) |
Column Temperature: | 25 ºC |
Flow Gradient: | 0.0-2.0 min: 96% B, 2.0-5.5 min: 88% B, 5.5- 8.5 min: 88% B, 8.5-9.0 min: 86% B, 9.0-14.0 min: 86% B, 14.0-17.0 min: 82% B, 17.0-23.0 min: 65% B, 23.0-24.0 min: 65%B, 24.0-24.5 min: 96% B, 24.5-29.0 min: 96% B |
Flow Rate: | 0.25 mL/min |
Solvent A: | 100% water, 10 mM ammonium acetate, 2.5 μM InfinityLab deactivator additive (Agilent, P-N. 5191-4506) |
Solvent B: | 15% water/85% acetonitrile, 10 mM ammonium acetate, 2.5 μM InfinityLab deactivator additive (Agilent, P-N. 5191-4506) |
Chromatography Type: | HILIC |
Chromatography ID: | CH003140 |
Instrument Name: | Agilent 1290 Infinity II |
Column Name: | Waters XBridge BEH Amide (100 x 2.1mm,2.5um) |
Column Temperature: | 25 ºC |
Flow Gradient: | 0.0-3.0min: 98% B, 3.0-6.0 min: 98% B, 6.0-11.0 min: 70% B, 11.0-12.0 min: 60%, 12.0-16.0 min: 5% B, 16.0-18.0 min: 5% B, 18.0-19.0 min: 98% B and 0.1% formic acid |
Flow Rate: | 0.25 mL/min |
Solvent A: | 100% water, 10 mM ammonium formate ,2.5 μM InfinityLab deactivator additive (Agilent, P-N. 5191-4506) and 0.1% formic acid |
Solvent B: | in 10% water/90% acetonitrile, 10 mM ammonium formate 2.5 μM InfinityLab deactivator additive (Agilent, P-N. 5191-4506) |
Chromatography Type: | HILIC |