Summary of Study ST000167
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000145. The data can be accessed directly via it's Project DOI: 10.21228/M87596 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000167 |
Study Title | Cold Storage of Rat Hepatocyte Spheroids |
Study Type | Storage condition testing |
Study Summary | The purpose of this study was to optimize conditions for cold storage of rat hepatocyte spheroids without freezing. Rat hepatocytes were isolated by a two-step perfusion method; hepatocyte spheroids were formed during 48 h of rocked culture in serum-free medium (SFM). Spheroids were then maintained in rocked culture at 37°C (control condition) or cold stored at 4°C for 24 or 48 h in six different cold storage solutions: SFM alone; SFM + 1 mM deferoxamine (Def); SFM + 1 ?M cyclosporin A (CsA); SFM + 1 mM Def + 1 ?M CsA, University of Wisconsin (UW) solution alone, UW + 1 mM Def. |
Institute | Mayo Clinic |
Department | Division of Experimental Surgery |
Last Name | Nyberg |
First Name | Scott |
Nyberg.scott@mayo.edu | |
Submit Date | 2015-05-14 |
Num Groups | 9 |
Total Subjects | 45 |
Raw Data Available | No |
Analysis Type Detail | LC-MS |
Release Date | 2015-06-28 |
Release Version | 1 |
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Subject:
Subject ID: | SU000186 |
Subject Type: | Animal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | SpragueDawley rats |
Weight Or Weight Range: | 300400 g |
Species Group: | Mammal |