Summary of Study ST000167

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000145. The data can be accessed directly via it's Project DOI: 10.21228/M87596 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000167
Study TitleCold Storage of Rat Hepatocyte Spheroids
Study TypeStorage condition testing
Study SummaryThe purpose of this study was to optimize conditions for cold storage of rat hepatocyte spheroids without freezing. Rat hepatocytes were isolated by a two-step perfusion method; hepatocyte spheroids were formed during 48 h of rocked culture in serum-free medium (SFM). Spheroids were then maintained in rocked culture at 37°C (control condition) or cold stored at 4°C for 24 or 48 h in six different cold storage solutions: SFM alone; SFM + 1 mM deferoxamine (Def); SFM + 1 ?M cyclosporin A (CsA); SFM + 1 mM Def + 1 ?M CsA, University of Wisconsin (UW) solution alone, UW + 1 mM Def.
Institute
Mayo Clinic
DepartmentDivision of Experimental Surgery
Last NameNyberg
First NameScott
EmailNyberg.scott@mayo.edu
Submit Date2015-05-14
Num Groups9
Total Subjects45
Raw Data AvailableNo
Analysis Type DetailLC-MS
Release Date2015-06-28
Release Version1
Scott Nyberg Scott Nyberg
https://dx.doi.org/10.21228/M87596
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Animal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA0090953320uM diazepam
SA0090961720uM diazepam
SA009097120uM diazepam
SA00909836Control group 120h
SA00909920Control group 120h
SA0091004Control group 120h
SA00910118Control group 72h
SA0091022Control group 72h
SA00910334Control group 72h
SA00910435Control group 96h
SA00910519Control group 96h
SA0091063Control group 96h
SA00910725SFM cold 24-120h
SA0091089SFM cold 24-120h
SA00910921SFM cold 24-96h
SA0091105SFM cold 24-96h
SA00911129SFM cold 48-120h
SA00911213SFM cold 48-120h
SA00911312SFM +CsA cold 24-120h
SA00911428SFM +CsA cold 24-120h
SA00911524SFM +CsA cold 24-96h
SA0091168SFM +CsA cold 24-96h
SA00911732SFM +CsA cold 48-120h
SA00911816SFM +CsA cold 48-120h
SA00911942SFM +CsA+Gly cold 24-120h
SA00912039SFM +CsA+Gly cold 24-96h
SA00912145SFM +CsA+Gly cold 48-120h
SA00913126SFM+Def cold 24-120h
SA00913210SFM+Def cold 24-120h
SA00913340SFM+Def cold 24-120h
SA00913422SFM+Def cold 24-96h
SA00913537SFM+Def cold 24-96h
SA0091366SFM+Def cold 24-96h
SA00913730SFM+Def cold 48-120h
SA00913843SFM+Def cold 48-120h
SA00913914SFM+Def cold 48-120h
SA00912227SFM +Def +CsA cold 24-120h
SA00912311SFM +Def +CsA cold 24-120h
SA00912441SFM +Def +CsA cold 24-120h
SA00912523SFM +Def +CsA cold 24-96h
SA0091267SFM +Def +CsA cold 24-96h
SA00912738SFM +Def +CsA cold 24-96h
SA00912831SFM +Def +CsA cold 48-120h
SA00912915SFM +Def +CsA cold 48-120h
SA00913044SFM +Def +CsA cold 48-120h
Showing results 1 to 45 of 45
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