Summary of Study ST000167
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000145. The data can be accessed directly via it's Project DOI: 10.21228/M87596 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000167 |
| Study Title | Cold Storage of Rat Hepatocyte Spheroids |
| Study Type | Storage condition testing |
| Study Summary | The purpose of this study was to optimize conditions for cold storage of rat hepatocyte spheroids without freezing. Rat hepatocytes were isolated by a two-step perfusion method; hepatocyte spheroids were formed during 48 h of rocked culture in serum-free medium (SFM). Spheroids were then maintained in rocked culture at 37°C (control condition) or cold stored at 4°C for 24 or 48 h in six different cold storage solutions: SFM alone; SFM + 1 mM deferoxamine (Def); SFM + 1 ?M cyclosporin A (CsA); SFM + 1 mM Def + 1 ?M CsA, University of Wisconsin (UW) solution alone, UW + 1 mM Def. |
| Institute | Mayo Clinic |
| Department | Division of Experimental Surgery |
| Last Name | Nyberg |
| First Name | Scott |
| Nyberg.scott@mayo.edu | |
| Submit Date | 2015-05-14 |
| Num Groups | 9 |
| Total Subjects | 45 |
| Raw Data Available | No |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-06-28 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR000192 |
| Treatment Summary: | UW alone|UW + 1 mM Def|SFM alone|SFM + 1 mM Def|SFM + 1 mM Def + 1 µM CsA|SFM + 1 µM CsA |
| Treatment Protocol Filename: | PMID-23507348-Liu-Nydberg-CellTrans-2014.pdf |
| Treatment Protocol Comments: | Treatment annotation Cold 24 Indicates 24 hours cold storate prior to treatment Cold 48 Indicates 48 hours cold storate prior to treatment -96h Indicates treated for 96 hours after cold storage -120h Indicates treated for 120 hours after cold storage |
| Treatment Compound: | University of Wisconsin (UW) solution|UW + deferoxamine (Def)| serum-free medium; Sigma-Aldrich (St. Louis, MO, USA)|SFM + Def|SFM + Def + cyclosporin A (CsA)|SFM + CsA |
