Summary of Study ST001149
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000768. The data can be accessed directly via it's Project DOI: 10.21228/M8DH49 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001149 |
Study Title | Plasmodium Niemann-Pick Type C1-Related Protein is a Druggable Target Required for Parasite Membrane Homeostasis |
Study Type | Drug Exposure |
Study Summary | Metabolic examination/confirmation of resistance mutations and GFP tagging observed in study |
Institute | Pennsylvania State University |
Department | BMB |
Laboratory | Llinás Laboratory |
Last Name | Llinás |
First Name | Manuel |
Address | W-122 Millennium Science Complex, Pollock Road |
eso5005@psu.edu | |
Phone | 8148673527 |
Submit Date | 2019-02-15 |
Raw Data Available | Yes |
Analysis Type Detail | LC-MS |
Release Date | 2019-05-15 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR001229 |
Treatment Summary: | Infected Red Blood cells were exposed to Antimalarial Compounds, associated with sample names. All samples were treated with 10xIC50 of a compound for 2.5 hours or left untreated for 2.5 hours. |
Treatment Protocol ID: | Metabolite_Extraction_for_LCMS_2017_Llinas |
Treatment Protocol Filename: | Metabolite_Extraction_for_LCMS_2017_Llinas.pdf |
Treatment: | Abiotic |
Treatment Compound: | ATQ, MMV009108, MMV019662, MMV028038 |
Treatment Route: | Addition to media |
Treatment Dose: | 10xIC50, 2-10xIC50 for archival data |
Treatment Dosevolume: | Always equal to or less than 10 uL per 5 mL sample |
Treatment Doseduration: | 2.5 hours |
Treatment Vehicle: | Stocks kept in either DMSO or Water |
Cell Storage: | Temperature/Gas Mixture controlled incubator |
Cell Growth Container: | 6-well plate |
Cell Growth Config: | 5 mL per well, each with 1x10^8 cells |
Cell Media: | RPMI 1640 w/ Albumax, Gentamycin, Hypoxanthine, HEPES, Sodium Bicarbonate |
Cell Harvesting: | PBS Wash followed by 90% methanol extraction |