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MB Sample ID: SA023598
| Local Sample ID: | Lemos_010 |
| Subject ID: | SU000487 |
| Subject Type: | Bacterial cells |
| Subject Species: | Streptococcus mutans |
| Taxonomy ID: | 1309 |
| Genotype Strain: | UA159 |
| Species Group: | Microorganism |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000487 |
| Subject Type: | Bacterial cells |
| Subject Species: | Streptococcus mutans |
| Taxonomy ID: | 1309 |
| Genotype Strain: | UA159 |
| Species Group: | Microorganism |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Lemos_010 | SA023598 | FL005698 | WT | Strain |
| Lemos_010 | SA023598 | FL005698 | 0.00 | Exposure to H2O2 |
Collection:
| Collection ID: | CO000481 |
| Collection Summary: | Upon reaching OD600 = 0.4, the culture is split into 4 samples. Three are treated with H2O2 (0.5 mM). The other is harvested by filtration onto 0.45uM nitrocellulose membrane. The cells will be scraped off of the membrane into ice-cold PBS (~1 mL), centrifuged for 30 seconds at 12K RPM, liquid aspirated and stored at -80C until sending for analysis. This process will be repeated with the remaining three samples after 15, 30 and 60 minutes treatment with H2O2. |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR000501 |
| Treatment Summary: | 0.5 mM H2O2 for 15, 30 and 60 minutes. Control has 0 H2O2 added. |
Sample Preparation:
| Sampleprep ID: | SP000494 |
| Sampleprep Summary: | Amino acids|Organic acids |
| Sampleprep Protocol Filename: | SB_AA_Assay.pdf SB_OA_Assay.pdf |
Combined analysis:
| Analysis ID | AN000728 | AN000729 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 6490 | Thermo Dionex Ultimate 3000 |
| Column | Waters AccQ Tag (100 x 2mm,1.7um) | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ | Thermo Quantiva QQQ |
| Ion Mode | POSITIVE | POSITIVE |
| Units | nmol/mg protein | nmol/mg protein |
Chromatography:
| Chromatography ID: | CH000521 |
| Chromatography Summary: | Amino acids |
| Methods Filename: | SB_AA_Assay.pdf |
| Instrument Name: | Agilent 6490 |
| Column Name: | Waters AccQ Tag (100 x 2mm,1.7um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.7 mL/min |
| Solvent A: | AccQ·Tag Eluent A |
| Solvent B: | accQ·Tag Eluent B |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000522 |
| Chromatography Summary: | Organic acids |
| Methods Filename: | SB_OA_Assay.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Column Temperature: | 45 |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000645 |
| Analysis ID: | AN000728 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000646 |
| Analysis ID: | AN000729 |
| Instrument Name: | Thermo Quantiva QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
