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MB Sample ID: SA023598
| Local Sample ID: | Lemos_010 |
| Subject ID: | SU000487 |
| Subject Type: | Bacterial cells |
| Subject Species: | Streptococcus mutans |
| Taxonomy ID: | 1309 |
| Genotype Strain: | UA159 |
| Species Group: | Microorganism |
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Collection:
| Collection ID: | CO000481 |
| Collection Summary: | Upon reaching OD600 = 0.4, the culture is split into 4 samples. Three are treated with H2O2 (0.5 mM). The other is harvested by filtration onto 0.45uM nitrocellulose membrane. The cells will be scraped off of the membrane into ice-cold PBS (~1 mL), centrifuged for 30 seconds at 12K RPM, liquid aspirated and stored at -80C until sending for analysis. This process will be repeated with the remaining three samples after 15, 30 and 60 minutes treatment with H2O2. |
| Sample Type: | Bacterial cells |
