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MB Sample ID: SA057228
| Local Sample ID: | WT7_72h_0mM_3 |
| Subject ID: | SU000994 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57Bl6 OT-1 |
| Age Or Age Range: | 6-8 weeks |
| Animal Animal Supplier: | Breeding at Moffitt for several generations, originally from Jax |
| Animal Light Cycle: | 12 hour |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000994 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57Bl6 OT-1 |
| Age Or Age Range: | 6-8 weeks |
| Animal Animal Supplier: | Breeding at Moffitt for several generations, originally from Jax |
| Animal Light Cycle: | 12 hour |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| WT7_72h_0mM_3 | SA057228 | FL010314 | 72 | Time activated with peptide |
| WT7_72h_0mM_3 | SA057228 | FL010314 | - | DFMO treatment (mM) |
Collection:
| Collection ID: | CO000988 |
| Collection Summary: | At 48 or 72 hours cells in culture medium were centrifuged on a Ficoll gradient to enrich viable cells. Cells were washed 1x with PBS, counted, and frozen as cell pellets at -80. |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR001008 |
| Treatment Summary: | Splenic CD8+ T cells were isolated from 4 OT-1 mice (WT1, 3, 7, 8) by negative immunomagnetic selection. T cells stimulated with ova-derived peptide (SIINFEKL) that binds with high affinity to the OT-1 transgenic TCR and 10ng/mL IL-2 with and without 5mM DFMO for 48-72 hours. Cell stoarge: 37 degrees, Cell growth container: 24 well plate, cell media: RPMI1640 with glutamine. Added components were: NEAA, Sodium Pyruvate, Fetal Bovine Serum, Fungizone, Gentimycin, Pen/strep. |
Sample Preparation:
| Sampleprep ID: | SP001001 |
| Sampleprep Summary: | None |
| Sampleprep Protocol Filename: | GMetabolomics_LCMS_Protocol_092117.pdf Appendix_A_Internal_Standard_Prep_GLCMS.pdf |
Combined analysis:
| Analysis ID | AN001566 | AN001567 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak height | peak height |
Chromatography:
| Chromatography ID: | CH001098 |
| Methods Filename: | GMetabolomics_LCMS_Protocol_092117.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 350 ul/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001444 |
| Analysis ID: | AN001566 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS001445 |
| Analysis ID: | AN001567 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
