Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA057228

Local Sample ID:	WT7_72h_0mM_3
Subject ID:	SU000994
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Genotype Strain:	C57Bl6 OT-1
Age Or Age Range:	6-8 weeks
Animal Animal Supplier:	Breeding at Moffitt for several generations, originally from Jax
Animal Light Cycle:	12 hour

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Treatment:

Treatment ID:	TR001008
Treatment Summary:	Splenic CD8+ T cells were isolated from 4 OT-1 mice (WT1, 3, 7, 8) by negative immunomagnetic selection. T cells stimulated with ova-derived peptide (SIINFEKL) that binds with high affinity to the OT-1 transgenic TCR and 10ng/mL IL-2 with and without 5mM DFMO for 48-72 hours. Cell stoarge: 37 degrees, Cell growth container: 24 well plate, cell media: RPMI1640 with glutamine. Added components were: NEAA, Sodium Pyruvate, Fetal Bovine Serum, Fungizone, Gentimycin, Pen/strep.

Treatment ID:

TR001008

Treatment Summary:

Splenic CD8+ T cells were isolated from 4 OT-1 mice (WT1, 3, 7, 8) by negative immunomagnetic selection. T cells stimulated with ova-derived peptide (SIINFEKL) that binds with high affinity to the OT-1 transgenic TCR and 10ng/mL IL-2 with and without 5mM DFMO for 48-72 hours. Cell stoarge: 37 degrees, Cell growth container: 24 well plate, cell media: RPMI1640 with glutamine. Added components were: NEAA, Sodium Pyruvate, Fetal Bovine Serum, Fungizone, Gentimycin, Pen/strep.