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MB Sample ID: SA128419
| Local Sample ID: | (9) WT 4h_009 |
| Subject ID: | SU001597 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001597 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| (9) WT 4h_009 | SA128419 | FL016025 | WT - 4h fast | Treamtent group |
Collection:
| Collection ID: | CO001592 |
| Collection Summary: | 2 to 5 drops of blood were taken from the facial vein of each mouse using sterile 5mm goldenrod animal lancets. Blood was allowed to clot for 30-80 minutes at room temperature, then centrifuged 1,000 x g for 15 min at 4°C, snap frozen with liquid nitrogen and stored at -80°C. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR001612 |
| Treatment Summary: | 4 genotypes/3 time points, 5-7, Genotypes: WT, Sirt1-OE, Sirt6-OE, Sirt1+6-OE Time points: Fed - 4h fast- 16h fast |
Sample Preparation:
| Sampleprep ID: | SP001605 |
| Sampleprep Summary: | 2 to 5 drops of blood were taken from the facial vein of each mouse using sterile 5mm goldenrod animal lancets. Blood was allowed to clot for 30-80 minutes at room temperature, then centrifuged 1,000 x g for 15 min at 4°C, snap frozen with liquid nitrogen and stored at -80°C. |
Combined analysis:
| Analysis ID | AN002541 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Leco Pegasus IV GC |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | QTOF |
| MS instrument name | Leco Pegasus IV TOF |
| Ion Mode | POSITIVE |
| Units | normalized peak height |
Chromatography:
| Chromatography ID: | CH001861 |
| Chromatography Summary: | primary metabolism by GCTOF |
| Instrument Name: | Leco Pegasus IV GC |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS002359 |
| Analysis ID: | AN002541 |
| Instrument Name: | Leco Pegasus IV TOF |
| Instrument Type: | QTOF |
| MS Type: | EI |
| MS Comments: | A Leco Pegasus IV time of flight mass spectrometer is controlled by the Leco ChromaTOF software vs. 2.32 (St. Joseph, MI). The transfer line temperature between gas chromatograph and mass spectrometer is set to 280°C. Electron impact ionization at 70V is employed with an ion source temperature of 250°C. Acquisition rate is 17 spectra/second, with a scan mass range of 85-500 Da. |
| Ion Mode: | POSITIVE |
