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MB Sample ID: SA174316
| Local Sample ID: | s3-1 |
| Subject ID: | SU001936 |
| Subject Type: | Invertebrate |
| Subject Species: | Drosophila melanogaster |
| Taxonomy ID: | 7227 |
| Age Or Age Range: | Developmentally-staged-larvae were collected (randomized) 4 days after placing male and female flies in fresh vials for a 12-hour period. |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001936 |
| Subject Type: | Invertebrate |
| Subject Species: | Drosophila melanogaster |
| Taxonomy ID: | 7227 |
| Age Or Age Range: | Developmentally-staged-larvae were collected (randomized) 4 days after placing male and female flies in fresh vials for a 12-hour period. |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| s3-1 | SA174316 | FL021383 | dTorKO Ctdnep1 | Genotype |
Collection:
| Collection ID: | CO001929 |
| Collection Summary: | Fat body tissues of the indicated genotypes were collected and stored at -80°C until processed for MS analysis. Quantitative MS analysis was performed on ten 4 day-old Drosophila larval fat bodies homogenized in 50µl D-PBS (Dulbecco’s Phosphate Buffered Saline without Mg2+ and Ca2+) by Lipotype using previously described methods (Grillet et al, 2016). |
| Sample Type: | Insect tissue |
Treatment:
| Treatment ID: | TR001948 |
| Treatment Summary: | In this study we did not apply any additional treatment(s) to the samples. |
Sample Preparation:
| Sampleprep ID: | SP001942 |
| Sampleprep Summary: | Lipids from fat body tissues of indicated genotypes were extracted using chloroform and methanol (1). Samples were spiked with lipid class-specific internal standards prior to extraction. After drying and resuspending in MS acquisition mixture, lipid extracts were subjected to mass spectrometric analysis. Sample preparations were done by Lipotype. (1) Sampaio, J. L. et al. Membrane lipidome of an epithelial cell line. Proceedings of the National Academy of Sciences 108, 1903–1907 (2011). |
Combined analysis:
| Analysis ID | AN003013 | AN003014 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | None (Direct infusion) | None (Direct infusion) |
| Chromatography system | none | none |
| Column | none | none |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | pmol | pmol |
Chromatography:
| Chromatography ID: | CH002233 |
| Chromatography Summary: | NA |
| Instrument Name: | none |
| Column Name: | none |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS002802 |
| Analysis ID: | AN003013 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Spectra acquisition Mass spectra were acquired on a hybrid quadrupole/Orbitrap mass spectrometer equipped with an automated nano-flow electrospray ion source in both positive and negative ion mode. Data processing and normalization Lipid identification using LipotypeXplorer (2) was performed on unprocessed (*.raw format) mass spectra. For MS-only mode, lipid identification was based on the molecular masses of the intact molecules. MSMS mode included the collision induced fragmentation of lipid molecules and lipid identification was based on both the intact masses and the masses of the fragments. Prior to normalization and further statistical analysis lipid identifications were filtered according to mass accuracy, occupation threshold, noise and background. Lists of identified lipids and their intensities were stored in a database optimized for the particular structure inherent to lipidomic datasets. Intensity of lipid class-specific internal standards was used for lipid quantification. (2) Herzog, R. et al. A novel informatics concept for high-throughput shotgun lipidomics based on the molecular fragmentation query language. Genome Biol. 12, R8 (2011). |
| Ion Mode: | POSITIVE |
| MS ID: | MS002803 |
| Analysis ID: | AN003014 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Spectra acquisition Mass spectra were acquired on a hybrid quadrupole/Orbitrap mass spectrometer equipped with an automated nano-flow electrospray ion source in both positive and negative ion mode. Data processing and normalization Lipid identification using LipotypeXplorer (2) was performed on unprocessed (*.raw format) mass spectra. For MS-only mode, lipid identification was based on the molecular masses of the intact molecules. MSMS mode included the collision induced fragmentation of lipid molecules and lipid identification was based on both the intact masses and the masses of the fragments. Prior to normalization and further statistical analysis lipid identifications were filtered according to mass accuracy, occupation threshold, noise and background. Lists of identified lipids and their intensities were stored in a database optimized for the particular structure inherent to lipidomic datasets. Intensity of lipid class-specific internal standards was used for lipid quantification. (2) Herzog, R. et al. A novel informatics concept for high-throughput shotgun lipidomics based on the molecular fragmentation query language. Genome Biol. 12, R8 (2011). |
| Ion Mode: | NEGATIVE |
