Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA174316

Local Sample ID:	s3-1
Subject ID:	SU001936
Subject Type:	Invertebrate
Subject Species:	Drosophila melanogaster
Taxonomy ID:	7227
Age Or Age Range:	Developmentally-staged-larvae were collected (randomized) 4 days after placing male and female flies in fresh vials for a 12-hour period.
Gender:	Male and female

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Sample Preparation:

Sampleprep ID:	SP001942
Sampleprep Summary:	Lipids from fat body tissues of indicated genotypes were extracted using chloroform and methanol (1). Samples were spiked with lipid class-specific internal standards prior to extraction. After drying and resuspending in MS acquisition mixture, lipid extracts were subjected to mass spectrometric analysis. Sample preparations were done by Lipotype. (1) Sampaio, J. L. et al. Membrane lipidome of an epithelial cell line. Proceedings of the National Academy of Sciences 108, 1903–1907 (2011).

Sampleprep ID:

SP001942

Sampleprep Summary:

Lipids from fat body tissues of indicated genotypes were extracted using chloroform and methanol (1). Samples were spiked with lipid class-specific internal standards prior to extraction. After drying and resuspending in MS acquisition mixture, lipid extracts were subjected to mass spectrometric analysis. Sample preparations were done by Lipotype. (1) Sampaio, J. L. et al. Membrane lipidome of an epithelial cell line. Proceedings of the National Academy of Sciences 108, 1903–1907 (2011).