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MB Sample ID: SA217539
Local Sample ID: | Chitosan-films_25cm2_01 |
Subject ID: | SU002353 |
Subject Type: | Food item |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002353 |
Subject Type: | Food item |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Chitosan-films_25cm2_01 | SA217539 | FL026416 | Chitosan-films_25cm2 | Group |
Collection:
Collection ID: | CO002346 |
Collection Summary: | After alcoholic fermentation, the wine was bottled in glass bottles (750 mL) under four different conditions: i) without preservation treatment (2 bottles); ii) addition of 40 mg/L of free SO2 (4 bottles); addition of iii) a Ch-Ge film with 100 cm2 surface area (5 bottles) and iv) a Ch-Ge film with 25 cm2 surface area (3 bottles). All wine samples were stored in the absence of light at 10-15 ºC, for 12 months. After opening the bottles, the wine samples were immediately prepared for NMR analysis. |
Sample Type: | Wine |
Treatment:
Treatment ID: | TR002365 |
Treatment Summary: | Not applicable |
Sample Preparation:
Sampleprep ID: | SP002359 |
Sampleprep Summary: | 100 µL of 1 M potassium phosphate (KH2PO4), with 0.1% sodium salt of 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid (TSP-d4, chemical shift reference) in D2O were added to 900 µL of wine. The wine samples pH (originally ca. pH 3.5) was adjusted to 3.0 ± 0.03 by adding 5% (v/v) HCl in D2O, and 600 µL of the mixture were transferred into 5 mm NMR tubes. |
Processing Method: | None |
Sample Resuspension: | None |
Sample Spiking: | 0.1% sodium salt of 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid (TSP-d4), as a chemical shift reference |
Analysis:
MB Sample ID: | SA217539 |
Analysis ID: | AN003704 |
Laboratory Name: | Metabolomics group |
Analysis Type: | NMR |
Operator Name: | Joao A. Rodrigues |
NMR:
NMR ID: | NM000247 |
Analysis ID: | AN003704 |
Instrument Name: | Bruker AVANCE III 500 spectrometer |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
Spectrometer Frequency: | 500 MHz |
NMR Probe: | TXI probe |
NMR Solvent: | 90% H2O/ 10% D2O |
NMR Tube Size: | 5 mm NMR tubes |
Shimming Method: | Topshim |
Pulse Sequence: | noesypr1d |
Water Suppression: | presat |
Pulse Width: | 90-degree |
Temperature: | 300 K |
Number Of Scans: | 128 |
Dummy Scans: | 16 |
Acquisition Time: | 3.3 s |
Relaxation Delay: | 2 s |
Num Data Points Acquired: | 32 k |
Line Broadening: | 0.3 Hz |
Zero Filling: | 64 k |
Baseline Correction Method: | manual |
Chemical Shift Ref Std: | 0 ppm for TSP-d4 |
NMR Results File: | 4_Wine_processed_data.txt UNITS:ppm |