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MB Sample ID: SA256278
| Local Sample ID: | GALT_NR14_21756609 |
| Subject ID: | SU002652 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002652 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| GALT_NR14_21756609 | SA256278 | FL032765 | Ctrl | Factor |
Collection:
| Collection ID: | CO002645 |
| Collection Summary: | Fifty-four DBS samples were collected from genetically and biochemically confirmed GAL (n = 15) patients at King Faisal Specialist Hospital and Research center (KFSHRC) and healthy controls (n = 39). |
| Collection Protocol Filename: | Characteristics of the study population and metabolites extraction |
| Sample Type: | Blood (plasma) |
Treatment:
| Treatment ID: | TR002664 |
| Treatment Summary: | no treatment use |
Sample Preparation:
| Sampleprep ID: | SP002658 |
| Sampleprep Summary: | Metabolites extraction The polar metabolites were extracted from DBS samples using our developed standard protocol (Jacob et al., 2018). Five 3 mm size DBS disks were used for metabolite extraction using methanol, acetonitrile, and water (40:40:20%) for protein precipitation. The mixture was mixed at 25°C and 600 rpm for 2 hours in a thermomixer (Eppendorf, Germany). Pooled QC samples were prepared using aliquots from the study samples. Afterward, the supernatants were transferred to another set of tubes, evaporated in SpeedVacc (Christ, City, Germany), and stored at −80°C until LCMS analysis. |
| Sampleprep Protocol Filename: | Metabolites extraction |
| Processing Storage Conditions: | -20℃ |
| Extract Storage: | Room temperature |
Combined analysis:
| Analysis ID | AN004202 | AN004203 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity | Waters Acquity |
| Column | Waters Acquity UPLC XSelect HSS C18 (100 × 2.1mm, 2.5um) | Waters Acquity UPLC XSelect HSS C18 (100 × 2.1mm, 2.5um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Waters Xevo-G2-S | Waters Xevo-G2-S |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH003114 |
| Methods Filename: | UPLCHRMS |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity UPLC XSelect HSS C18 (100 × 2.1mm, 2.5um) |
| Column Temperature: | 55 |
| Flow Gradient: | 0–16 min 95%–5% A, 16–19 min 5% A, 19–20 min 5%–95% A, and 20–22 min, 95%– 95% A |
| Flow Rate: | 300 μl/min. |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 50% methanol/50% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003949 |
| Analysis ID: | AN004202 |
| Instrument Name: | Waters Xevo-G2-S |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The DIA data were collected with a Masslynx™ V4.1 workstation in continuum mode (Waters Inc., Milford, MA, USA). The raw MS data were processed following a standard pipeline using the Progenesis QI v.3.0 software. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003950 |
| Analysis ID: | AN004203 |
| Instrument Name: | Waters Xevo-G2-S |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The DIA data were collected with a Masslynx™ V4.1 workstation in continuum mode (Waters Inc., Milford, MA, USA). The raw MS data were processed following a standard pipeline using the Progenesis QI v.3.0 software. |
| Ion Mode: | NEGATIVE |
