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MB Sample ID: SA256278

Local Sample ID:GALT_NR14_21756609
Subject ID:SU002652
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Male and female

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Sample Preparation:

Sampleprep ID:SP002658
Sampleprep Summary:Metabolites extraction The polar metabolites were extracted from DBS samples using our developed standard protocol (Jacob et al., 2018). Five 3 mm size DBS disks were used for metabolite extraction using methanol, acetonitrile, and water (40:40:20%) for protein precipitation. The mixture was mixed at 25°C and 600 rpm for 2 hours in a thermomixer (Eppendorf, Germany). Pooled QC samples were prepared using aliquots from the study samples. Afterward, the supernatants were transferred to another set of tubes, evaporated in SpeedVacc (Christ, City, Germany), and stored at −80°C until LCMS analysis.
Sampleprep Protocol Filename:Metabolites extraction
Processing Storage Conditions:-20℃
Extract Storage:Room temperature