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MB Sample ID: SA079884

Local Sample ID:FFS118C
Subject ID:SU001216
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:19 - 26 yr
Weight Or Weight Range:50.6 - 94.1 kg
Height Or Height Range:1.6 - 1.85 m
Gender:Male and female
Human Smoking Status:Non-smoker
Human Exclusion Criteria:Subjects with anemia, diabetes, thyroid disease, MetS, cancer, previous cardiovascular events or other disease diagnoses were excluded. Subjects were also excluded if they had extreme dietary or exercise patterns, or were taking prescription medications or other supplements known to alter lipoprotein metabolism such as isoflavones.

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Combined analysis:

Analysis ID AN001899
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6550 QTOF
Ion Mode NEGATIVE
Units ug/ml

Chromatography:

Chromatography ID:CH001375
Methods Filename:zivkovic_protocol.pdf
Instrument Name:Waters Acquity
Column Name:Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
Column Temperature:65°C
Flow Gradient:0 min 15% (B), 0–2 min 30% (B), 2–2.5 min 48% (B), 2.5–11 min 82% (B), 11–11.5 min 99% (B), 11.5–12 min 99% (B), 12–12.1 min 15% (B), 12.1–15 min 15% (B)
Flow Rate:0.6 mL/min
Injection Temperature:4°C
Solvent A:40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate
Solvent B:90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate
Chromatography Type:Reversed phase
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