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MB Sample ID: SA079884
| Local Sample ID: | FFS118C |
| Subject ID: | SU001216 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 19 - 26 yr |
| Weight Or Weight Range: | 50.6 - 94.1 kg |
| Height Or Height Range: | 1.6 - 1.85 m |
| Gender: | Male and female |
| Human Smoking Status: | Non-smoker |
| Human Exclusion Criteria: | Subjects with anemia, diabetes, thyroid disease, MetS, cancer, previous cardiovascular events or other disease diagnoses were excluded. Subjects were also excluded if they had extreme dietary or exercise patterns, or were taking prescription medications or other supplements known to alter lipoprotein metabolism such as isoflavones. |
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Combined analysis:
| Analysis ID | AN001899 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6550 QTOF |
| Ion Mode | NEGATIVE |
| Units | ug/ml |
MS:
| MS ID: | MS001755 |
| Analysis ID: | AN001899 |
| Instrument Name: | Agilent 6550 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Data are analyzed in a four-stage process. First, raw data are processed in an untargeted (qualitative) manner by Agilent’s software MassHunter Qual to find peaks in up to 300 chromatograms. Peak features are then imported into MassProfilerProfessional for peak alignments to seek which peaks are present in multiple chromatograms, using exclusion criteria by the minimum percentage of chromatograms in which these peaks are positively detected. We usually use 30% as minimum criterion. In a tedious manual process, these peaks are then collated and constrained into a MassHunter quantification method on the accurate mass precursor ion level, using the MS/MS information and the LipidBlast library to identify lipids with manual confirmation of adduct ions and spectral scoring accuracy. MassHunter enables back-filling of quantifications for peaks that were missed in the primary peak finding process, hence yielding data sets without missing values. |
| Ion Mode: | NEGATIVE |
