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MB Sample ID: SA098895
| Local Sample ID: | VV_14_HEpG2_C2 |
| Subject ID: | SU001433 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | HPACC |
| Cell Passage Number: | 10-20 |
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Combined analysis:
| Analysis ID | AN002263 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Ultimate 3000 |
| Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | Normalised peak area |
Chromatography:
| Chromatography ID: | CH001662 |
| Chromatography Summary: | The mobile phases consisted of 60:40 ACN: H2O with 10mM ammonium formate, 0.1% formic acid and 5µM of phosphoric acid (A) and 90:10 IPA:ACN with 10 mM ammonium formate, 0.1% formic acid and 5µM phosphoric acid (B). The gradient was as follows: 0-2 min 30% (B); 2-8 min 50% (B); 8-15 min 99% (B), 15-16 min 99% (B), 16-17 min 30% (B). Sample temperature was maintained at 6°C in the autosampler and 5 µL of sample were injected into the LC-MS instrument. |
| Instrument Name: | Ultimate 3000 |
| Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| Flow Gradient: | 0-2 min 30% (B); 2-8 min 50% (B); 8-15 min 99% (B), 15-16 min 99% (B), 16-17 min 30% (B). |
| Solvent A: | 60% acetonitrile/40% water; 0.1% formic acid; 10mM ammonium formate; 5µM phosphoric acid |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate;5µM phosphoric acid |
| Chromatography Type: | Reversed phase |
