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MB Sample ID: SA098895
| Local Sample ID: | VV_14_HEpG2_C2 |
| Subject ID: | SU001433 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | HPACC |
| Cell Passage Number: | 10-20 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002263 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Ultimate 3000 |
| Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | Normalised peak area |
MS:
| MS ID: | MS002107 |
| Analysis ID: | AN002263 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Thermo Q-Exactive Orbitrap MS instrument was operated in both positive and negative polarities, using the following parameters: mass range 240-1200 m/z (positive) and 240-1600 (negative), spray voltage 3.8kV (ESI+) and 3kV (ESI-), sheath gas (nitrogen) flow rate 60 units, auxiliary gas (nitrogen) flow rate 25 units, capillary temperature (320°C), full scan MS1 mass resolving power 70,000. Data dependent fragmentation (dd-MS/MS) parameters for each polarity as follows: TopN: 10, resolution 17,500 units, maximum injection time: 25 ms, automatic gain control target: 5e5 and normalised collision energy of 20 and 25 (arbitrary units) in positive polarity. TopN: 5, resolution 17,500 units, maximum injection time: 80 ms automatic gain control target: 5e5 and normalised collision energy of 20 and 30 (arbitrary units) in negative polarity. The instrument was externally calibrated to <1ppm using ESI positive and negative calibration solutions (Thermo Fisher Scientific). |
| Ion Mode: | POSITIVE |
