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MB Sample ID: SA242785
| Local Sample ID: | Heavy_Pool-P03 |
| Subject ID: | SU002516 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003950 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE |
| Units | unitless L/H ratio |
Chromatography:
| Chromatography ID: | CH002924 |
| Chromatography Summary: | A Thermo Scientific Vanquish Horizon UHPLC System coupled with a Thermo Scientific Q Exactive HF Orbitrap was used to conduct LC-(+)FTMS analysis. A Waters ACQUITY UPLC BEH C18 (1.7 μm, 2.1 x 100 mm) column was used along with the following mobile phases: (A) 0.1% formic acid in water and (B) 0.1% formic acid in acetonitrile. The gradient was 25% B (0 min), 25% B (1.2 min), 99% B (11.2 min), 99% B (16.2 min), 25% B (16.3 min), and 25% B (19 min) to achieve column equilibration. The column was maintained at 40 ºC with flowrate of 400 μL/min. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | 25% B (0 min), 25% B (1.2 min), 99% B (11.2 min), 99% B (16.2 min), 25% B (16.3 min), 25% B (19 min) |
| Flow Rate: | 400 uL/min |
| Solvent A: | 100% water, 0.1% formic acid |
| Solvent B: | 100% acetonitrile, 0.1% formic acid |
| Chromatography Type: | Reversed phase |
