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MB Sample ID: SA015478
| Local Sample ID: | AB33_U_7 |
| Subject ID: | SU000373 |
| Subject Type: | Plant |
| Subject Species: | Cynodon transvaalensis |
| Taxonomy ID: | 75728 |
| Genotype Strain: | AB03, AB33, and AB39 |
| Age Or Age Range: | 4 month old plant clones |
| Subject Comments: | (African Bermudagrass,C. transvaalensis) |
| Species Group: | Plant |
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Collection:
| Collection ID: | CO000367 |
| Collection Summary: | Rinsed roots were cut off the plant, placed into paper envelopes, and immediately placed into liquid nitrogen. Roots were stored at -80°C until freeze-drying. Freeze-dried roots were held at room temperature in ziploc bags with silica gel packets. They were then frozen in liquid nitrogen and ground to a powder in a tissue homogenizer with ball bearings (Geno/Grinder 2010), and then stored in 2 ml snap-cap plastic tubes at room temperature. |
| Sample Type: | plant root tissue |
| Collection Method: | whole plant destruction |
| Collection Location: | greenhouse |
| Collection Frequency: | once |
| Collection Duration: | 1-2 minutes to cut off and rinse the roots prior to placement into liquid nitrogen |
| Collection Time: | 136 days after inoculation |
| Volumeoramount Collected: | 0.2-0.5 grams of root tissue, dry weight |
| Storage Conditions: | 1)-80C until freeze-drying. 2) Held at room temperature in ziploc bags with silica packets. 3) Frozen in liquid nitrogen and ground using a Geno/Grinder 2010. 4) Transferred to 2 mL tubes and held at room temperature. |
| Collection Vials: | Paper coin envelopes |
| Storage Vials: | 2 ml snap-cap plastic tubes |
| Collection Tube Temp: | liquid nitrogen (-196°C) |
| Additives: | none |
