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MB Sample ID: SA015478
Local Sample ID: | AB33_U_7 |
Subject ID: | SU000373 |
Subject Type: | Plant |
Subject Species: | Cynodon transvaalensis |
Taxonomy ID: | 75728 |
Genotype Strain: | AB03, AB33, and AB39 |
Age Or Age Range: | 4 month old plant clones |
Subject Comments: | (African Bermudagrass,C. transvaalensis) |
Species Group: | Plant |
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Treatment:
Treatment ID: | TR000387 |
Treatment Summary: | Plants clones propagated by stolons were allowed to establish for 30 days in 4 cm diameter conetainers filled with sand. Fifty sting nematodes (mixed age and gender) were added to each conetainer and allowed to grow and reproduce for 90 days. Nematodes were then rinsed off of roots for quantification, and plant roots were frozen in liquid nitrogen and stored at -80C until they were freeze-dried.Freeze-dried roots were ground to a powder and then submitted for global metabolomic analysis. |
Treatment: | Biotic |
Treatment Compound: | Sting nematode, Belonolaimus longicaudatus |
Plant Growth Support: | sand in conetainers- 4 cm wide, 30 cm deep |
Plant Growth Location: | temperature controlled greenhouse |
Plant Plot Design: | complete randomized design |
Plant Light Period: | Natural lighting, ca. 11:13 Light:Dark |
Plant Humidity: | ca.50%RH |
Plant Temp: | 25°C |
Plant Watering Regime: | Twice daily for 5 min |
Plant Nutritional Regime: | Weekly liquid fertilizer of 24-8-16 (N-P-K) |
Plant Estab Date: | 2015-09-24 |
Plant Harvest Date: | 2016-04-02 |
Plant Growth Stage: | non-reproductive |
Plant Metab Quench Method: | liquid nitrogen, followed by storage at -80°C |
Plant Harvest Method: | rinsed roots were cut off the plant and placed into paper envelopes |
Plant Storage: | 1)-80C until freeze-drying. 2) Held at room temperature in ziploc bags with silica packets. 3) Frozen in liquid nitrogen and ground using a Geno/Grinder 2010. 4) Transferred to 2 mL tubes and held at room temperature. |