Return to study ST001721 main page

MB Sample ID: SA162038

Local Sample ID:FH5_2
Subject ID:SU001798
Subject Type:Invertebrate
Subject Species:Cherax tenuimanus
Taxonomy ID:99755
Weight Or Weight Range:116g-198g
Gender:Male and female

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN002804 AN002805
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS
Column Thermo Hypersil GOLD C18 (100 x 2.1mm,1.9m) Thermo Hypersil GOLD C18 (100 x 2.1mm,1.9m)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units peak area values peak area values

MS:

MS ID:MS002599
Analysis ID:AN002804
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Full scans with data-dependent tandem mass spectrometry were acquired on the Orbitrap mass analyzer. Full scans were acquired at a resolution of 70,000 at mass-to-charge ratio (m/z) 200 over the m/z range 70–1000 with the ESI conditions as follows: source heater = 350°C, sheath gas = 35 (arbitrary units), auxiliary gas = 10 (arbitrary units), capillary temperature 350°C, ion spray voltage = 3.0 kV (positive ion mode) and 2.5 kV (negative ion mode), S-lens 50%, and automatic gain control = 110¬¬¬-6. Tandem mass spectrometry experiments were performed at a resolution of 17,500 at m/z 200 on each sample with the higher energy collisional dissociation energy set at 20 eV. Data acquisition was carried out using Xcalibur software (Thermo Fisher Scientific). Before analysis, the Orbitrap was externally calibrated using ready-made calibration solutions (ESI-negative ion calibration and ESI-positive ion calibration solutions) obtained from Thermo Fisher Scientific.
Ion Mode:POSITIVE
Analysis Protocol File:Methods_Lette_marron
  
MS ID:MS002600
Analysis ID:AN002805
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Full scans with data-dependent tandem mass spectrometry were acquired on the Orbitrap mass analyzer. Full scans were acquired at a resolution of 70,000 at mass-to-charge ratio (m/z) 200 over the m/z range 70–1000 with the ESI conditions as follows: source heater = 350°C, sheath gas = 35 (arbitrary units), auxiliary gas = 10 (arbitrary units), capillary temperature 350°C, ion spray voltage = 3.0 kV (positive ion mode) and 2.5 kV (negative ion mode), S-lens 50%, and automatic gain control = 110¬¬¬-6. Tandem mass spectrometry experiments were performed at a resolution of 17,500 at m/z 200 on each sample with the higher energy collisional dissociation energy set at 20 eV. Data acquisition was carried out using Xcalibur software (Thermo Fisher Scientific). Before analysis, the Orbitrap was externally calibrated using ready-made calibration solutions (ESI-negative ion calibration and ESI-positive ion calibration solutions) obtained from Thermo Fisher Scientific.
Ion Mode:NEGATIVE
  logo