Return to study ST001911 main page
MB Sample ID: SA176930
Local Sample ID: | OB_12_A(raw) |
Subject ID: | SU001989 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | wildtype |
Age Or Age Range: | 4 weeks |
Gender: | Male |
Animal Animal Supplier: | Jackson Laboratory |
Animal Housing: | UC Denver |
Animal Feed: | High fat diet (HFD), 60 kcal% fat from Research diets Inc., # D12492 |
Animal Water: | ad libitum |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP001995 |
Sampleprep Summary: | Frozen plasma was stored at -80°C until sample preparation for untargeted liquid-chromatography mass spectrometry (LC-MS)-based metabolomics. Briefly, 100µL of sample underwent protein precipitation using methanol, followed by liquid-liquid extraction using methyl-tert butyl ether as previously described[1, 2] to obtain an aqueous fraction and a lipid fraction. The lipid fraction was dried down and resuspended in 100% methanol for LC-MS analysis. |
Processing Storage Conditions: | Described in summary |