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MB Sample ID: SA176932
| Local Sample ID: | OB+BMJ_05(raw) |
| Subject ID: | SU001989 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | wildtype |
| Age Or Age Range: | 4 weeks |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Laboratory |
| Animal Housing: | UC Denver |
| Animal Feed: | High fat diet (HFD), 60 kcal% fat from Research diets Inc., # D12492 |
| Animal Water: | ad libitum |
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Treatment:
| Treatment ID: | TR002001 |
| Treatment Summary: | Sixteen-week-old diet-induced obese (DIO) C57BL/6J male mice [fed-high fat diet (HFD), 60 kcal% fat from Research diets Inc., # D12492) from 4 weeks of age] were purchased from Jackson labs (Bar Harbor, ME). Once received at the animal house facility in UC Denver, the mice were randomized into two groups (n=4/ group): a) Control-DIO group maintained on the 60 kcal% HFD for additional 40 days, and b) BMJ+DIO group maintained on 60 kcal% HFD fat for additional 40 days plus orally gavage with lyophilized bitter melon juice extract [(5mg per mouse; total volume of 100 μl, 200mg/kg body weight/ day), reconstituted in sterile deionized water immediately before use)] throughout the study. |
| Treatment: | Intervention |
| Treatment Compound: | Bitter melon juice |
| Treatment Route: | Oral gavage |
| Treatment Dose: | 200mg/kg body weight/ day |
| Animal Fasting: | On day 40 of study initiation, mice in control and treament groups were subjected to fasting for six hours prior to sacrifice. |
| Animal Endp Euthanasia: | On day 40, mice were sacrificed after CO2 asphyxiation and exsanguination. |
