Summary of Study ST000494
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000371. The data can be accessed directly via it's Project DOI: 10.21228/M83C78 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000494 |
| Study Title | Timecourse of palmitate isotopomers in LNCaP Cells |
| Study Summary | Timecourse of palmitate isotopomers in LNCaP Cell pellets |
| Institute | Mayo Clinic |
| Laboratory | Mayo Clinic Metabolomics Resource Core |
| Last Name | Frigo |
| First Name | Daniel |
| Address | Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204-5001 |
| frigo@uh.edu | |
| Phone | 832-842-8824 |
| Submit Date | 2016-10-21 |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-10-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000371 |
| Project DOI: | doi: 10.21228/M83C78 |
| Project Title: | Metabolic Dissection of Aberrant Glutamine Metabolism in Prostate Cancer |
| Project Type: | MS targeted analysis |
| Project Summary: | In this project, we propose to define the specific role(s) of three crucial oncogenic signaling cascades (androgen receptor, Myc and AMPK) in regulating glutamine metabolism in prostate cancer using 13C-labeled glutamine with mass spectrometry-based metabolic flux analyses. |
| Institute: | Mayo Clinic |
| Laboratory: | Mayo Clinic Metabolomics Resource Core |
| Last Name: | Frigo |
| First Name: | Daniel |
| Address: | Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204-5001 |
| Email: | frigo@uh.edu |
| Phone: | 832-842-8824 |
Subject:
| Subject ID: | SU000515 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment | 13C media incubation time |
|---|---|---|---|
| SA025616 | ms5457-10 | treated-100 pM R1881 | 24 hours |
| SA025617 | ms5457-2 | treated-100 pM R1881 | 24 hours |
| SA025618 | ms5457-6 | treated-100 pM R1881 | 72 hours |
| SA025619 | ms5457-14 | treated-100 pM R1881 | 72 hours |
| SA025612 | ms5457-12 | treated-10 nM R1881 | 24 hours |
| SA025613 | ms5457-4 | treated-10 nM R1881 | 24 hours |
| SA025614 | ms5457-16 | treated-10 nM R1881 | 72 hours |
| SA025615 | ms5457-8 | treated-10 nM R1881 | 72 hours |
| SA025608 | ms5457-3 | treated-1 nM R1881 | 24 hours |
| SA025609 | ms5457-11 | treated-1 nM R1881 | 24 hours |
| SA025610 | ms5457-15 | treated-1 nM R1881 | 72 hours |
| SA025611 | ms5457-7 | treated-1 nM R1881 | 72 hours |
| SA025620 | ms5457-9 | treated-vehicle (ethanol) | 24 hours |
| SA025621 | ms5457-1 | treated-vehicle (ethanol) | 24 hours |
| SA025622 | ms5457-5 | treated-vehicle (ethanol) | 72 hours |
| SA025623 | ms5457-13 | treated-vehicle (ethanol) | 72 hours |
| Showing results 1 to 16 of 16 |
Collection:
| Collection ID: | CO000509 |
| Collection Summary: | LNCaP cell model |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR000529 |
| Treatment Summary: | LNCaP cells that were serum starved and incubated with 2mM [U-13C]glutamine and treated with dose response of a synthetic androgen (R1881) for 24 or 72 hours. |
Sample Preparation:
| Sampleprep ID: | SP000522 |
| Sampleprep Summary: | Add 50 uL 1x PBS solution to each vial containing cells. Cap, vortex, sonicate 2 x 20 sec bursts with vortexing inbetween and after Transfer entire contents of each vial into a labeled 1 dram vial. use 50 uL media for samples 9-16 use 50 uL plasma for QC Add 1.5 mL CHM solution to vial Add 20 uL 1:20 phenol red indicator Cap and vortex on multivortexer for 10 minutes Centrifuge @ 4C @ 3000 rpm for 10 minutes Aspirate and discard top phase hard to see the phase separation due to the light color (yellow). Dry bottom phase in speed vac Reconstitute samples and standards with 25 uL buffer A. (do triplicate injections) |
Combined analysis:
| Analysis ID | AN000760 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity |
| Column | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6460 QQQ |
| Ion Mode | UNSPECIFIED |
| Units | 13C-palmitate % enrichment |
Chromatography:
| Chromatography ID: | CH000546 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000672 |
| Analysis ID: | AN000760 |
| Instrument Name: | Agilent 6460 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | UNSPECIFIED |
