Summary of Study ST000494

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000371. The data can be accessed directly via it's Project DOI: 10.21228/M83C78 This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000494
Study Title	Timecourse of palmitate isotopomers in LNCaP Cells
Study Summary	Timecourse of palmitate isotopomers in LNCaP Cell pellets
Institute	Mayo Clinic
Laboratory	Mayo Clinic Metabolomics Resource Core
Last Name	Frigo
First Name	Daniel
Address	Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204-5001
Email	frigo@uh.edu
Phone	832-842-8824
Submit Date	2016-10-21
Analysis Type Detail	LC-MS
Release Date	2018-10-10
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000522
Sampleprep Summary:	Add 50 uL 1x PBS solution to each vial containing cells. Cap, vortex, sonicate 2 x 20 sec bursts with vortexing inbetween and after Transfer entire contents of each vial into a labeled 1 dram vial. use 50 uL media for samples 9-16 use 50 uL plasma for QC Add 1.5 mL CHM solution to vial Add 20 uL 1:20 phenol red indicator Cap and vortex on multivortexer for 10 minutes Centrifuge @ 4C @ 3000 rpm for 10 minutes Aspirate and discard top phase hard to see the phase separation due to the light color (yellow). Dry bottom phase in speed vac Reconstitute samples and standards with 25 uL buffer A. (do triplicate injections)

Sampleprep ID:

SP000522

Sampleprep Summary:

Add 50 uL 1x PBS solution to each vial containing cells. Cap, vortex, sonicate 2 x 20 sec bursts with vortexing inbetween and after Transfer entire contents of each vial into a labeled 1 dram vial. use 50 uL media for samples 9-16 use 50 uL plasma for QC Add 1.5 mL CHM solution to vial Add 20 uL 1:20 phenol red indicator Cap and vortex on multivortexer for 10 minutes Centrifuge @ 4C @ 3000 rpm for 10 minutes Aspirate and discard top phase hard to see the phase separation due to the light color (yellow). Dry bottom phase in speed vac Reconstitute samples and standards with 25 uL buffer A. (do triplicate injections)