Summary of Study ST001674

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001077. The data can be accessed directly via it's Project DOI: 10.21228/M8GT3N This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST001674
Study Title	Untargeted phospholipid analysis of HeLa silenced for or overexpressing GOLPH3
Study Summary	A group of sequentially-acting enzymes operating at the branchpoint among sphingolipid synthetic pathways binds the Golgi-localised oncoprotein GOLPH3. GOLPH3 sorts these enzymes into vesicles for intra-Golgi retro-transport, acting as a component of the cisternae inter-conversion mechanisms. Through these effects, GOLPH3 controls the sub-Golgi localisation, and the lysosomal degradation rate of specific enzymes. Here we evaluated the impact of overexpressing or silencing GOLPH3 on the glycerophospholipid composition of HeLa cells by untargeted lipid analysis.
Institute	École polytechnique fédérale de Lausanne (EPFL)
Department	IBI
Laboratory	UPDANGELO
Last Name	D'Angelo
First Name	Giovanni
Address	Station 15, Lausanne, Vaud, 1015, Switzerland
Email	giovanni.dangelo@epfl.ch
Phone	+41 216934276
Submit Date	2021-01-29
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2021-02-26
Release Version	1

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Combined analysis:

Analysis ID	AN002732
Analysis type	MS
Chromatography type	HILIC
Chromatography system	Shimadzu Prominence UFPLC xr system
Column	HILIC Kinetex (50 x 2.1mm,2.6um)
MS Type	ESI
MS instrument type	Orbitrap
MS instrument name	Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap
Ion Mode	POSITIVE
Units	intensities