Summary of Study ST001674
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001077. The data can be accessed directly via it's Project DOI: 10.21228/M8GT3N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001674 |
| Study Title | Untargeted phospholipid analysis of HeLa silenced for or overexpressing GOLPH3 |
| Study Summary | A group of sequentially-acting enzymes operating at the branchpoint among sphingolipid synthetic pathways binds the Golgi-localised oncoprotein GOLPH3. GOLPH3 sorts these enzymes into vesicles for intra-Golgi retro-transport, acting as a component of the cisternae inter-conversion mechanisms. Through these effects, GOLPH3 controls the sub-Golgi localisation, and the lysosomal degradation rate of specific enzymes. Here we evaluated the impact of overexpressing or silencing GOLPH3 on the glycerophospholipid composition of HeLa cells by untargeted lipid analysis. |
| Institute | École polytechnique fédérale de Lausanne (EPFL) |
| Department | IBI |
| Laboratory | UPDANGELO |
| Last Name | D'Angelo |
| First Name | Giovanni |
| Address | Station 15, Lausanne, Vaud, 1015, Switzerland |
| giovanni.dangelo@epfl.ch | |
| Phone | +41 216934276 |
| Submit Date | 2021-01-29 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-02-26 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002732 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Shimadzu Prominence UFPLC xr system |
| Column | HILIC Kinetex (50 x 2.1mm,2.6um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap |
| Ion Mode | POSITIVE |
| Units | intensities |
Chromatography:
| Chromatography ID: | CH002018 |
| Chromatography Summary: | Lipid extracts (2 μL injection volume in ChCl3:MeOH 2:1) were separated over an 8 minutes gradient at a flow rate of 200 μL/min on a HILIC Kinetex Column (2.6lm, 2.1 × 50 mm2) on a Shimadzu Prominence UFPLC xr system (Tokyo, Japan). Mobile phase A was acetonitrile:methanol 10:1 (v/v) containing 10 mM ammonium formate and 0.5% formic acid while mobile phase B was deionized water containing 10 mM ammonium formate and 0.5% formic acid. The elution of the gradient began with 5% B at a 200 μL/min flow and increased linearly to 50% B over 7 min, then the elution continued at 50% B for 1.5 min and finally, the column was re-equilibrated for 2.5 min. |
| Instrument Name: | Shimadzu Prominence UFPLC xr system |
| Column Name: | HILIC Kinetex (50 x 2.1mm,2.6um) |
| Flow Gradient: | The elution of the gradient began with 5% B and increased linearly to 50% B over 7 min, then the elution continued at 50% B for 1.5 min and finally, the column was re-equilibrated for 2.5 min. |
| Flow Rate: | 200 µL/min |
| Solvent A: | 91% acetonitrile/9% methanol; 0.5% formic acid; 10 mM ammonium formate |
| Solvent B: | 1005 water; 0.5% formic acid; 10 mM ammonium formate |
| Chromatography Type: | HILIC |
