Summary of Study ST002556

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001648. The data can be accessed directly via it's Project DOI: 10.21228/M8PF0K This work is supported by NIH grant, U2C- DK119886.


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Study IDST002556
Study TitleBlood metabolomics and impacted cellular mechanisms during transition into lactation in dairy cows that develop metritis
Study TypeCase-Control Study
Study SummaryThe objective of this study was to identify metabolites associated with metritis and use them for identification of cellular mechanisms affected during transition into lactation. Holstein cows (n = 104) had blood collected in the prepartum period (d-14 ± 6), at calving (d0), and at the day of metritis diagnosis (d7 ± 2). Cows with reddish or brownish, watery, and fetid discharge were diagnosed with metritis (n = 52). Cows with metritis were paired with herdmates without metritis (n = 52) based on DIM. The metabolome of plasma samples was evaluated using untargeted gas chromatography time-of-flight mass spectrometry. Univariate analyses included t-tests and fold change analyses. Metabolites with false discovery rate (FDR) adjusted P ≤ 0.10 on t-tests were used for partial least squares – discriminant analysis PLS-DA coupled with permutational analysis using 2,000 permutations. Metabolites with FDR adjusted P ≤ 0.10 on t-tests were also used for enriched pathway analyses and identification of cellular processes. Cows that developed metritis had affected cellular processes associated with lower amino acid metabolism in the prepartum period, greater lipolysis, cell death, and oxidative stress at calving and at metritis diagnosis, and greater leukocyte activation at calving, but lower immune cell activation at metritis diagnosis. In summary, cows that developed metritis had plasma metabolomic changes associated with greater lipolysis, oxidative stress, and a dysregulated immune response which may predispose cows to metritis development.
University of Florida
DepartmentCollege of Veterinary Medicine
LaboratoryLarge Animal Clinical Sciences
Last Namesegundocasaro
First NameSegundo
Address117 Deriso Hall, 2015 SW 16th Ave.
Submit Date2023-04-11
Num Groups2
Total Subjects104
Num Females104
Analysis Type DetailGC-MS
Release Date2023-04-28
Release Version1
Segundo segundocasaro Segundo segundocasaro application/zip

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Combined analysis:

Analysis ID AN004211
Analysis type MS
Chromatography type GC
Chromatography system Leco Pegasus IV GC
Column Restek Rtx-5Sil MS (30m x 0.25mm, 0.25um)
MS Type EI
MS instrument type GC-TOF
MS instrument name Leco Pegasus IV TOF
Units peak heights