Summary of Study ST002556

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001648. The data can be accessed directly via it's Project DOI: 10.21228/M8PF0K This work is supported by NIH grant, U2C- DK119886.


Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)
Study IDST002556
Study TitleBlood metabolomics and impacted cellular mechanisms during transition into lactation in dairy cows that develop metritis
Study TypeCase-Control Study
Study SummaryThe objective of this study was to identify metabolites associated with metritis and use them for identification of cellular mechanisms affected during transition into lactation. Holstein cows (n = 104) had blood collected in the prepartum period (d-14 ± 6), at calving (d0), and at the day of metritis diagnosis (d7 ± 2). Cows with reddish or brownish, watery, and fetid discharge were diagnosed with metritis (n = 52). Cows with metritis were paired with herdmates without metritis (n = 52) based on DIM. The metabolome of plasma samples was evaluated using untargeted gas chromatography time-of-flight mass spectrometry. Univariate analyses included t-tests and fold change analyses. Metabolites with false discovery rate (FDR) adjusted P ≤ 0.10 on t-tests were used for partial least squares – discriminant analysis PLS-DA coupled with permutational analysis using 2,000 permutations. Metabolites with FDR adjusted P ≤ 0.10 on t-tests were also used for enriched pathway analyses and identification of cellular processes. Cows that developed metritis had affected cellular processes associated with lower amino acid metabolism in the prepartum period, greater lipolysis, cell death, and oxidative stress at calving and at metritis diagnosis, and greater leukocyte activation at calving, but lower immune cell activation at metritis diagnosis. In summary, cows that developed metritis had plasma metabolomic changes associated with greater lipolysis, oxidative stress, and a dysregulated immune response which may predispose cows to metritis development.
University of Florida
DepartmentCollege of Veterinary Medicine
LaboratoryLarge Animal Clinical Sciences
Last Namesegundocasaro
First NameSegundo
Address117 Deriso Hall, 2015 SW 16th Ave.
Submit Date2023-04-11
Num Groups2
Total Subjects104
Num Females104
Analysis Type DetailGC-MS
Release Date2023-04-28
Release Version1
Segundo segundocasaro Segundo segundocasaro application/zip

Select appropriate tab below to view additional metadata details:


Subject type: Mammal; Subject species: Bos taurus (Factor headings shown in green)

mb_sample_id local_sample_id Group Time Parity
SA2568989794_Calving_ConCon Calving Mult
SA2568999778_Calving_ConCon Calving Mult
SA2569009979_Calving_ConCon Calving Mult
SA2569019971_Calving_ConCon Calving Mult
SA2569029802_Calving_ConCon Calving Mult
SA2569039966_Calving_ConCon Calving Mult
SA2569049804_Calving_ConCon Calving Mult
SA2569059536_Calving_ConCon Calving Mult
SA2569069985_Calving_ConCon Calving Mult
SA2569079280_Calving_ConCon Calving Mult
SA2569089268_Calving_ConCon Calving Mult
SA2569099945_Calving_ConCon Calving Mult
SA25691010001_Calving_ConCon Calving Mult
SA2569119992_Calving_ConCon Calving Mult
SA2569129823_Calving_ConCon Calving Mult
SA2569139987_Calving_ConCon Calving Mult
SA2569149509_Calving_ConCon Calving Mult
SA25691510038_Calving_ConCon Calving Mult
SA2569169924_Calving_ConCon Calving Mult
SA2569179918_Calving_ConCon Calving Mult
SA2569189917_Calving_ConCon Calving Mult
SA2569199925_Calving_ConCon Calving Mult
SA2569209927_Calving_ConCon Calving Mult
SA2569219957_Calving_ConCon Calving Mult
SA2569229944_Calving_ConCon Calving Mult
SA2569239950_Calving_ConCon Calving Mult
SA2569249942_Calving_ConCon Calving Mult
SA2569259954_Calving_ConCon Calving Mult
SA2569269956_Calving_ConCon Calving Mult
SA2569279875_Calving_ConCon Calving Mult
SA2569289872_Calving_ConCon Calving Mult
SA2569299904_Calving_ConCon Calving Mult
SA2569309876_Calving_ConCon Calving Mult
SA2569319907_Calving_ConCon Calving Mult
SA25693210303_Calving_ConCon Calving Prim
SA25693310264_Calving_ConCon Calving Prim
SA25693410254_Calving_ConCon Calving Prim
SA25693510212_Calving_ConCon Calving Prim
SA25693610246_Calving_ConCon Calving Prim
SA25693710262_Calving_ConCon Calving Prim
SA25693810268_Calving_ConCon Calving Prim
SA25693910274_Calving_ConCon Calving Prim
SA25694010311_Calving_ConCon Calving Prim
SA25694110261_Calving_ConCon Calving Prim
SA25694210229_Calving_ConCon Calving Prim
SA25694310290_Calving_ConCon Calving Prim
SA25694410245_Calving_ConCon Calving Prim
SA25694510194_Calving_ConCon Calving Prim
SA25694610171_Calving_ConCon Calving Prim
SA25694710165_Calving_ConCon Calving Prim
SA25694810199_Calving_ConCon Calving Prim
SA25694910189_Calving_ConCon Calving Prim
SA2569509985_Diagnosis_ConCon Diagnosis Mult
SA2569519904_Diagnosis_ConCon Diagnosis Mult
SA2569529907_Diagnosis_ConCon Diagnosis Mult
SA2569539918_Diagnosis_ConCon Diagnosis Mult
SA2569549954_Diagnosis_ConCon Diagnosis Mult
SA2569559917_Diagnosis_ConCon Diagnosis Mult
SA2569569872_Diagnosis_ConCon Diagnosis Mult
SA2569579876_Diagnosis_ConCon Diagnosis Mult
SA2569589804_Diagnosis_ConCon Diagnosis Mult
SA2569599794_Diagnosis_ConCon Diagnosis Mult
SA2569609778_Diagnosis_ConCon Diagnosis Mult
SA2569619536_Diagnosis_ConCon Diagnosis Mult
SA2569629802_Diagnosis_ConCon Diagnosis Mult
SA2569639268_Diagnosis_ConCon Diagnosis Mult
SA2569649509_Diagnosis_ConCon Diagnosis Mult
SA2569659823_Diagnosis_ConCon Diagnosis Mult
SA2569669280_Diagnosis_ConCon Diagnosis Mult
SA2569679875_Diagnosis_ConCon Diagnosis Mult
SA2569689925_Diagnosis_ConCon Diagnosis Mult
SA2569699979_Diagnosis_ConCon Diagnosis Mult
SA2569709971_Diagnosis_ConCon Diagnosis Mult
SA2569719987_Diagnosis_ConCon Diagnosis Mult
SA25697210038_Diagnosis_ConCon Diagnosis Mult
SA2569739992_Diagnosis_ConCon Diagnosis Mult
SA2569749924_Diagnosis_ConCon Diagnosis Mult
SA25697510001_Diagnosis_ConCon Diagnosis Mult
SA2569769966_Diagnosis_ConCon Diagnosis Mult
SA2569779944_Diagnosis_ConCon Diagnosis Mult
SA2569789942_Diagnosis_ConCon Diagnosis Mult
SA2569799927_Diagnosis_ConCon Diagnosis Mult
SA2569809957_Diagnosis_ConCon Diagnosis Mult
SA2569819945_Diagnosis_ConCon Diagnosis Mult
SA2569829956_Diagnosis_ConCon Diagnosis Mult
SA2569839950_Diagnosis_ConCon Diagnosis Mult
SA25698410254_Diagnosis_ConCon Diagnosis Prim
SA25698510194_Diagnosis_ConCon Diagnosis Prim
SA25698610171_Diagnosis_ConCon Diagnosis Prim
SA25698710189_Diagnosis_ConCon Diagnosis Prim
SA25698810261_Diagnosis_ConCon Diagnosis Prim
SA25698910165_Diagnosis_ConCon Diagnosis Prim
SA25699010199_Diagnosis_ConCon Diagnosis Prim
SA25699110212_Diagnosis_ConCon Diagnosis Prim
SA25699210245_Diagnosis_ConCon Diagnosis Prim
SA25699310262_Diagnosis_ConCon Diagnosis Prim
SA25699410229_Diagnosis_ConCon Diagnosis Prim
SA25699510274_Diagnosis_ConCon Diagnosis Prim
SA25699610303_Diagnosis_ConCon Diagnosis Prim
SA25699710311_Diagnosis_ConCon Diagnosis Prim
Showing page 1 of 4     Results:    1  2  3  4  Next     Showing results 1 to 100 of 312