Summary of Study ST000025

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000024. The data can be accessed directly via it's Project DOI: 10.21228/M85P4V This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000025
Study TitleMetabolomics Analysis of Thermally Challenged Mayfly Larvae (GCMS analysis)
Study TypeMetabolomic analysis of mayflies
Study SummaryThe purpose of this study was to examine the metabolic profiles of mayfly (Centroptilum triangulifer) larvae subjected to thermal challenge. This species is unusual in terms of its ease of culture, and its suitability as a laboratory test organism. Our purpose here was to examine how an environmentally realistic thermal challenge affects the physiology of this organism. In this study, we obtained several types of insect species and we were able to show that GC-MS Metabolomics could be used to distinguish among the different types of larvae.
University of North Carolina
DepartmentSystems and Translational Sciences
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Submit Date2014-02-28
Num Groups7
Total Subjects13
Raw Data AvailableYes
Uploaded File Size870 M
Analysis Type DetailGC-MS
Release Date2015-03-12
Release Version1
Susan Sumner Susan Sumner application/zip

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Combined analysis:

Analysis ID AN000045
Analysis type MS
Chromatography type GC
Chromatography system Leco Pegasus IV GC
Column Restek Rtx-5Sil (30m x 0.25mm,0.25um)
MS Type EI
MS instrument type GC x GC-TOF
MS instrument name Leco Pegasus IV TOF
Units peak area


Chromatography ID:CH000026
Chromatography Summary:GC/MS analysis is based on Oliver Fiehn methods
Instrument Name:Leco Pegasus IV GC
Column Name:Restek Rtx-5Sil (30m x 0.25mm,0.25um)
Column Pressure:10.4 psi
Column Temperature:50C(0.5 min)-330C(@20C/min)-330C(5 min)
Flow Gradient:constant flow
Flow Rate:1.0 mL/min
Injection Temperature:250C
Retention Index:FAMEs markers (C8-C30)
Retention Time:FAMEs markers (C8-C30)
Analytical Time:20 minutes
Oven Temperature:50C(0.5 min)-330C(@20C/min)-330C(5 min)
Transferline Temperature:280C
Sample Syringe Size:10 L
Chromatography Type:GC