Summary of Study ST000025
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000024. The data can be accessed directly via it's Project DOI: 10.21228/M85P4V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000025 |
| Study Title | Metabolomics Analysis of Thermally Challenged Mayfly Larvae (GCMS analysis) |
| Study Type | Metabolomic analysis of mayflies |
| Study Summary | The purpose of this study was to examine the metabolic profiles of mayfly (Centroptilum triangulifer) larvae subjected to thermal challenge. This species is unusual in terms of its ease of culture, and its suitability as a laboratory test organism. Our purpose here was to examine how an environmentally realistic thermal challenge affects the physiology of this organism. In this study, we obtained several types of insect species and we were able to show that GC-MS Metabolomics could be used to distinguish among the different types of larvae. |
| Institute | University of North Carolina |
| Department | Systems and Translational Sciences |
| Laboratory | Sumner Lab |
| Last Name | Sumner |
| First Name | Susan |
| Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
| susan_sumner @unc.edu | |
| Phone | 704-250-5066 |
| Submit Date | 2014-02-28 |
| Num Groups | 7 |
| Total Subjects | 13 |
| Raw Data Available | Yes |
| Uploaded File Size | 870 M |
| Analysis Type Detail | GC-MS |
| Release Date | 2015-03-12 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000045 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Leco Pegasus IV GC |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC x GC-TOF |
| MS instrument name | Leco Pegasus IV TOF |
| Ion Mode | POSITIVE |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH000026 |
| Chromatography Summary: | GC/MS analysis is based on Oliver Fiehn methods |
| Instrument Name: | Leco Pegasus IV GC |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Column Pressure: | 10.4 psi |
| Column Temperature: | 50C(0.5 min)-330C(@20C/min)-330C(5 min) |
| Flow Gradient: | constant flow |
| Flow Rate: | 1.0 mL/min |
| Injection Temperature: | 250C |
| Retention Index: | FAMEs markers (C8-C30) |
| Retention Time: | FAMEs markers (C8-C30) |
| Analytical Time: | 20 minutes |
| Oven Temperature: | 50C(0.5 min)-330C(@20C/min)-330C(5 min) |
| Transferline Temperature: | 280C |
| Sample Syringe Size: | 10 L |
| Chromatography Type: | GC |
