Summary of Study ST000025

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000024. The data can be accessed directly via it's Project DOI: 10.21228/M85P4V This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000025
Study TitleMetabolomics Analysis of Thermally Challenged Mayfly Larvae (GCMS analysis)
Study TypeMetabolomic analysis of mayflies
Study SummaryThe purpose of this study was to examine the metabolic profiles of mayfly (Centroptilum triangulifer) larvae subjected to thermal challenge. This species is unusual in terms of its ease of culture, and its suitability as a laboratory test organism. Our purpose here was to examine how an environmentally realistic thermal challenge affects the physiology of this organism. In this study, we obtained several types of insect species and we were able to show that GC-MS Metabolomics could be used to distinguish among the different types of larvae.
University of North Carolina
DepartmentSystems and Translational Sciences
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Submit Date2014-02-28
Num Groups7
Total Subjects13
Raw Data AvailableYes
Uploaded File Size870 M
Analysis Type DetailGC-MS
Release Date2015-03-12
Release Version1
Susan Sumner Susan Sumner application/zip

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Sample Preparation:

Sampleprep ID:SP000038
Sampleprep Summary:Insect samples homogenized in 50% acetonitrile at a concentration of 50 mg/mL. Sample dried and aliquot taken for NMR. Sample returned and extract processed for GC/MS analysis. Samples were derivatized using 2 step derivatization method (methoximation with methoxy amine followed by TMS derivatization with MSTFA).
Sampleprep Protocol Filename:RTI_DB_INSECT_Metabolomics_Procedure_March12_2014.docx
Processing Method:Homogenization and Solvent Removal with SpeedVac
Processing Storage Conditions:4C
Extraction Method:50% Acetonitrile
Extract Concentration Dilution:dried on SpeedVac
Extract Storage:-80C
Sample Resuspension:resuspended in D2O + Chenomix ISTD solution
Sample Derivatization:Methoximation with methoxy amine at 40 mg/ml in pyridine followed by TMS derivatization with MSTFA
Sample Spiking:FAMES (C8-C30) added to MSTFA as retention time markers